164610 | p-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem

164610
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      Empirical FormulaCAS #
      C₈H₉HgNO₂ 6283-24-5

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      RéférenceDisponibilité Conditionnement Qté Prix Quantité
      164610-1SET
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          Boîte carton 1 set
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          164610-700MG
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              Plastic Bag(s) 700 mg
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              Description
              OverviewAn organomercurial agent used for the activation of latent MMPs in vitro. Acts by facilitating the loss of the enzyme propeptide domain through an autolytic cleavage known as the cysteine switch.
              Note: 1 set = 5 x 700 mg.
              Catalogue Number164610
              Brand Family Calbiochem®
              SynonymsAPMA
              References
              ReferencesChen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113.
              Marcy, A.I., et al. 1991. Biochemistry 30, 6476.
              Stricklin, G.P., et al. 1983. Biochemistry 22, 61.
              Product Information
              CAS number6283-24-5
              ATP CompetitiveN
              FormYellow solid
              Hill FormulaC₈H₉HgNO₂
              Chemical formulaC₈H₉HgNO₂
              ReversibleN
              Structure formula ImageStructure formula Image
              Applications
              Biological Information
              Primary TargetActivation of latent MMPs in vitro
              Purity≥90% by titration
              Physicochemical Information
              Cell permeableN
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSOV5550000
              Safety Information
              R PhraseR: 26/27/28-36/37/38

              Very toxic by inhalation, in contact with skin and if swallowed.
              Irritating to eyes, respiratory system and skin.
              S PhraseS: 36

              Wear suitable protective clothing.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Highly Toxic
              Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
              Storage +15°C to +30°C
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 month at 4°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              FDS

              Titre

              Fiche de données de sécurité des matériaux (FDS) 

              Certificats d'analyse

              TitreNuméro de lot
              164610

              Références bibliographiques

              Aperçu de la référence bibliographique
              Chen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113.
              Marcy, A.I., et al. 1991. Biochemistry 30, 6476.
              Stricklin, G.P., et al. 1983. Biochemistry 22, 61.
              Fiche technique

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision06-September-2007 RFH
              SynonymsAPMA
              DescriptionAn organomercurial agent used for the activation of latent matrix metalloproteinases (MMPs) in vitro. Acts by facilitating the loss of the enzyme propeptide domain through an autolytic cleavage known as the cysteine switch.
              FormYellow solid
              Recommended reaction conditionsThe MMPs are members of a family of at least twenty Zn2+-dependent endopeptidases that function extracellularly at neutral pH. The MMPs are initially synthesized as latent or inactive zymogens that contain a regulatory propeptide. This propeptide confers the latency by binding to the Zn2+ at the enzyme's active site. MMP activation can be achieved by autoactivation and by the action of other MMPs or proteases, such as furin, urokinase, plasmin, and trypsin, as well as by organomercurial compounds. The following is a protocol from Stricklin, et al. (1983. Biochemistry 22, 61) describing the use of paminophenylmercuric acetate (APMA) to activate pro-MMPs. This protocol is good for other types of organomercurials such as p-(hydroxymercuric) benzoate (PHMB), phenylmercuric chloride (PMC), and mersalyl. Organomercurial stock solutions Prepare stock solutions in 0.1 M NaOH at a concentration range of 10-50 mM just prior to use. Although not absolutely necessary, the stock solution may be adjusted to pH 11 with 5 N HCl. (e.g. Marcy, A.I. et al. 1991. Biochemistry 30, 6476). ProMMPs Prepare proenzyme solutions in 0.1 M Tris-HCl, pH 7.5 at 1-2 mg/ml. Activation of proMMPs To initiate activation, combine proMMP solution with organomercurial stock in a 10:1 volume* ratio (MMPs to organomercurial). Incubate the mixture at 37°C for 2-3 hrs**. *When more organomercurial is desired, make the stock solution more concentrated. Do not exceed the above ratio so that the volume of organomercurial in the mixture is more than about 1/10 of that of the proMMP solution; this could result in significant changes in pH. **It is recommended that an analytical run be conducted first to determine the optimal incubation time. For example, a small-scale experiment with fixed concentration of proMMP and organomercurial would be incubated as described above. Remove aliquots of sample at various time points. Stop the reaction by the addition of concentrated SDS-sample buffer (for example, add 10 µl of 2X sample buffer to a 10 µl aliquot or add 2 µl of 5X sample buffer to an 8 µl aliquot) and then boil. The progress of the activation can then be monitored qualitatively by analyzing these time aliquots on a 12% SDS-PAGE gel. Removing organomercurial from the mixture after activation The activated MMPs can be used without the removal of the organomercurial. Researchers are referred to the following reference for the removal of organomercurial by gel filtration if desired: Marcy, A.I., et al. 1991. Biochemistry 30, 6476
              CAS number6283-24-5
              RTECSOV5550000
              Chemical formulaC₈H₉HgNO₂
              Structure formulaStructure formula
              Purity≥90% by titration
              SolubilityDMSO (10 mg/ml) or 0.1 N NaOH (10 mg/ml)
              Storage +15°C to +30°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 month at 4°C.
              Toxicity Highly Toxic
              ReferencesChen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113.
              Marcy, A.I., et al. 1991. Biochemistry 30, 6476.
              Stricklin, G.P., et al. 1983. Biochemistry 22, 61.