MABE917-25UL Anti-DNA G-quadruplex structures Antibody, clone BG4

MABE917-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HGel Electrophoresis, ICC, ChIP-seq, ELISAE. coliPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE917-25UL
      DescriptionAnti-DNA G-quadruplex structures Antibody, clone BG4
      Alternate Names
      • DNA G-quadruplex (BG4)
      Background InformationDNA G-quadruplex structures are associated with a number of important aspects of genome function, which include transcription, recombination and replication. Single-stranded guanine (G)-rich DNA can form stable secondary structures called G-quadruplexes (G-tetrads or G4), which can influence methylation at CpG islands (CGIs). G4 structure is reported to be tightly associated with CGI hypomethylation in the human genome. Four guanine bases can associate through Hoogsteen hydrogen bonding to form a square planar structure called a guanine tetrad (G-tetrad), and two or more G-tetrads can stack on top of each other to form a G-quadruplex. The quadruplex structure is further stabilized by the presence of a cation, especially potassium, which sits in a central channel between each pair of tetrads. A DNA G-tetrad can be formed within one DNA strand (intramolecular), between two DNA strands (bimolecular), or four DNA strands (tetramolecular). G4 DNA can arise anywhere in the genome where sufficiently long stretches of single-stranded G-rich DNA are exposed during replication, transcription or recombination. Chemical analysis of quadruplex-forming oligonucleotides has revealed the existence of a plethora of dynamic quadruplex structures with varying stabilities. The diverse nature of G4 DNA structures makes it an attractive topic in molecular biomedical research.(Ref.: Mao, SQ et al., (2018). Nat. Struc. Mol. Biol. 25(10); 951-957).
      References
      Product Information
      FormatPurified
      PresentationPurified monoclonal antibody in PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-DNA G-quadruplex structures, clone BG4 Antibody, Cat. No. MABE917-25UL, is a highly specific e. coli monoclonal antibody, that targets and has been tested in Gel Electrophoresis, Immunocytochemistry, ChIP-seq and ELISA.
      Key Applications
      • Gel Electrophoresis
      • Immunocytochemistry
      • ChIP-seq
      • ELISA
      Application NotesELISA Analysis: A representative lot detected DNA G-quadruplex structures in Anti-DNA G-quarduplex structures, BG4 (C ourtesy of University of Cambridge). ChIP-seq Analysis: A representative lot detected DNA G-quadruplex structures in ChIP-seq applications (Hansel-Hertsch, R., et. al. (2016). Nat Genet. 48(10):1267-72). ELISA Analysis: A representative lot detected DNA G-quadruplex structures in ELISA applications (Biffi, G., et. al. (2013). Nat Chem. 5(3):182-6). Immunocytochemistry Analysis: A representative lot detected DNA G-quadruplex structures in Immunocytochemistry applications (Biffi, G., et. al. (2013). Nat Chem. 5(3):182-6).
      Biological Information
      ImmunogenG-quadruplex oligonucleotides.
      ConcentrationPlease refer to lot specific datasheet.
      HostE. coli
      SpecificityClone BG4 has a high affinity for intramolecular and intermolecular DNA G-quadruplexes with no detectable binding to a RNA hairpin, single-stranded DNA or double-stranded DNA.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Purification MethodImmobilized Metal Affinity Chromatography (IMAC)
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe Anti-DNA-G-quadruplex structures, clone BG4 antibody  ( Cat. No. MABE917)  was resolved by SDS-PAGE, transferred to PVDF membrane and probed with Anti-FLAG epitope tag antibody (MAB3118).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications