MAB5434 | Anti-Oct-1 Antibody, clone YL15

MAB5434
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5434
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Oct-1 Antibody, clone YL15
      Background InformationOct proteins belong to the POU domain transcription factor family (for review see, Herr et al. (1995) Genes and Dev. 9:1679-1693). POU domain is a bipartite module containing POU homeo and POU specific subdomains, identified in more than 30 nuclear proteins that recognize relative cis-elements in promoters, enhancers and silencers of many genes. Oct-1 and Oct-2 proteins recognize the octamer consensus sequence ATGCAAAT and with lower affinity for oct-related sequences.
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in PBS containing 0.1% sodium azide.
      Applications
      ApplicationDetect Oct -1 with Anti-Oct-1 Antibody, clone YL15 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern blot: 2.5 μg/mL. Recognizes the ~96 kDa protein.

      Immunocytochemistry: 1-2μg/mL

      Immunoprecipitation: 2-5 μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenFull length recombinant human Oct-1 protein.
      CloneYL15
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with Oct-1. Recognizes a ~96kDa protein in nuclear extracts of HeLa cells.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • POU2F1
      • OCT1
      • NF-A1
      • OTF1
      • OTF-1
      • Oct-1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14859 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity).
      SIZE: 743 amino acids; 76472 Da
      SUBUNIT: Interacts with NR3C1, AR, PGR and HCFC1.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitous. Isoform 2 is lymphocyte-specific.
      PTM: Phosphorylated by PRKDC.
      SIMILARITY: SwissProt: P14859 ## Belongs to the POU transcription factor family. Class- 2 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-OCT-1 MONOCLONAL ANTIBODY - 2379334 2379334
      MOUSE ANTI-OCT-1 MONOCLONAL ANTIBODY - 2398872 2398872
      MOUSE ANTI-OCT-1 MONOCLONAL ANTIBODY - 2433521 2433521
      MOUSE ANTI-OCT-1 -2789765 2789765
      MOUSE ANTI-OCT-1 MONOCLONAL ANTIBODY 2979940

      References

      Reference overviewApplicationSpeciesPub Med ID
      A protease-independent function for SPPL3 in NFAT activation.
      Makowski, SL; Wang, Z; Pomerantz, JL
      Molecular and cellular biology  35  451-67  2015

      Show Abstract
      25384971 25384971
      Gender-specific potential inhibitory role of Ca2+/calmodulin dependent protein kinase phosphatase (CaMKP) in pressure-overloaded mouse heart.
      Prévilon, M; Pezet, M; Vinet, L; Mercadier, JJ; Rouet-Benzineb, P
      PloS one  9  e90822  2014

      Show Abstract
      Western BlottingMouse24608696 24608696
      Transcription factor oct1 is a somatic and cancer stem cell determinant.
      Jessica Maddox,Arvind Shakya,Samuel South,Dawne Shelton,Jared N Andersen,Stephanie Chidester,Jinsuk Kang,Keith M Gligorich,David A Jones,Gerald J Spangrude,Bryan E Welm,Dean Tantin
      PLoS genetics  8  2012

      Show Abstract
      23144633 23144633
      Characterization and functional analysis of the 5'-flanking promoter region of the mouse Tcf3 gene.
      Nina Solberg,Ondrej Machon,Stefan Krauss
      Molecular and cellular biochemistry  360  2012

      Show Abstract
      21935611 21935611
      Dissociation of Oct-1 from the nuclear peripheral structure induces the cellular aging-associated collagenase gene expression
      Imai, S, et al
      Mol Biol Cell, 8:2407-19 (1997)  1997

      ImmunocytochemistryHuman9398664 9398664
      The POU domain: versatility in transcriptional regulation by a flexible two-in-one DNA-binding domain
      Herr, W and Cleary, M A
      Genes Dev, 9:1679-93 (1995)  1995

      7622033 7622033
      Ethidium bromide provides a simple tool for identifying genuine DNA-independent protein associations.
      Lai, J S and Herr, W
      Proc. Natl. Acad. Sci. U.S.A., 89: 6958-62 (1992)  1992

      1495986 1495986

      Data Sheet

      Title
      Anti-Oct-1, clone YL15 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies