SCR035 Rodent Neuron Differentiation Kit

SCR035
1 kit  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      IF, CULT
      Description
      Catalogue NumberSCR035
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionRodent Neuron Differentiation Kit
      Background InformationThe Rodent Neuron Differentiation Kit is a system designed for the optimal differentiation of neural stem cells derived from rodents into neurons. Included in the kit are two neural inducers that when added to the Neural Stem Cell Basal Medium (Catalogue No. SCM003) allows for the preferential differentiation of rodent neural stem cells to a neuronal lineage. Other components of the kit include reagents for the fixation and two antibodies for the immunocytochemical characterization of the resulting neuron population (mouse anti-βIII-tubulin for neurons and rabbit anti-GFAP for astrocytes).
      Materials Required but Not DeliveredNeural Stem Cell Basal Medium (Cat. No. SCM003)
      Rodent neural stem cells (Cat. No. SCR022, SCR029, SCR031)
      References
      Product Information
      Components
      • Sufficient reagents are supplied to provide for 100 separate reactions at 1 mL volume or 10 reactions at 10 mL volume.
      • Neural Inducer A Solution (200 μL)
      • Neural Inducer B Solution (100 μL)
      • Mouse anti-β-III Tubulin monoclonal (50 μL)
      • Rabbit anti-GFAP polyclonal (50 μL)
      • Fixative (100 mL)
      Applications
      ApplicationThe Rodent Neuron Differentiation Kit is a system designed for the optimal differentiation of neural stem cells derived from rodents into neurons.
      Key Applications
      • Immunofluorescence
      • Cell Culture
      Biological Information
      Species Reactivity
      • Mouse
      • Rat
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe kit has been extensively validated on mouse cortical and spinal cord neural stem cells and on rat hippocampal neural stem cells. Using the Rodent Neuron Differentiation Kit, relatively pure populations of neurons (~70% βIII-tubulin-positive) are obtained. Very low levels of astroglial cells (<0.5%) are detected.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Rodent Neuron Differentiation Kit Certificates of Analysis

      TitleLot Number
      RODENT NEURON DIFFERENTIATION KIT - 23970792397079
      RODENT NEURON DIFFERENTIATION KIT - 24363542436354
      RODENT NEURON DIFFERENTIATION KIT - 19922691992269
      RODENT NEURON DIFFERENTIATION KIT - 20440942044094
      RODENT NEURON DIFFERENTIATION KIT - 20530102053010
      RODENT NEURON DIFFERENTIATION KIT - 21507172150717

      Technical Info

      Title
      MilliTrace Constitutive GFP Reporter Neural Stem Cell Lines

      Data Sheet

      Title
      RODENT NEURON DIFFERENTIATION KIT - Data Sheet