AB1778 Anti-Calbindin D-28K Antibody

AB1778
100 µL  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R ICC, IHC, IH(P), WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB1778
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Calbindin D-28K Antibody
      Alternate Names
      • Calbindin D28
      • RTVL-H protein
      • Vitamin D-dependent calcium-binding protein, avian-type 3
      • calbindin
      • calbindin 1, (28kD)
      • calbindin 1, 28kDa
      Background InformationCalbindin D-28k is a member of a large family of intracellular calcium-binding proteins of the EF-hand related to calmodulin and troponin-C. The biological roles of Calbindin D-28k include, calcium regulation (including calcium transport and uptake, calcification of bone and teeth) and calcium related signalling in neurons and transiently in embryological development. This calbindin also has a role in protecting neurons from apoptotic cell death. Disregulation of this calbindin protein relates to several disease states. Decline in calbindin D-28k in hippocampal dentate granule cells correlates with the kindling model for epilepsy. Calbindin D-28k immunoreactivity is specifically absent from neuronal poplulations lost early in ALS, while substantial loss of neurons which do contain the protein occur in patients with Huntington's disease.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse brain lysates and rat small intestine lysates.
      PresentationPurified rabbit polyclonal in phosphate buffered saline containing an antibody that has been affinity preabsorbed against calretinin. Contains no preservative.
      Applications
      ApplicationThis Anti-Calbindin D-28K Antibody is validated for use in IC, IH, IH(P), WB for the detection of Calbindin D-28K.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry:
      1:1000-1:5000. See online protocols.

      Immunohistochemistry(paraffin):

      Optimal working dilutions and protocols must be determined by the end user.
      Biological Information
      ImmunogenRecombinant mouse calbindin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityCalbindin. Shows no cross-reactivity to calretinin by Western blot. The antibody produces specific staining of cerebellum Purkinje cells, molecular layer dendrites and axonal fibers, stains cell bodies and fibers in neuronal subpopulations.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteMouse. Due to the homology of the protein it is expected to react with human, rat, and most species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCalbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted.
      Gene Symbol
      • CAB27
      • CALB
      • D-28K
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05937 # Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
      SIZE: 261 amino acids; 30025 Da
      SUBUNIT: Interacts with RANBP9.
      DOMAIN: SwissProt: P05937 This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
      SIMILARITY: Belongs to the calbindin family. & Contains 5 EF-hand domains.
      Molecular Weight28 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

      Western Blot Analysis: 1:1000 dilution of this lot detected CALBINDIN on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 uL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Calbindin D-28K2453654
      Anti-Calbindin D-28K - 21366032136603
      Anti-Calbindin D-28K - 23874322387432
      Anti-Calbindin D-28K - 23903912390391
      Anti-Calbindin D-28K - 24303242430324
      Anti-Calbindin D-28K - 19401431940143
      Anti-Calbindin D-28K - 19908961990896
      Anti-Calbindin D-28K - 20106432010643
      Anti-Calbindin D-28K - 20403762040376
      Anti-Calbindin D-28K - 22243822224382

      Referencias bibliográficas

      Visión general referenciasAplicación EspeciePub Med ID
      Neurovascular crosstalk between interneurons and capillaries is required for vision.
      Usui, Y; Westenskow, PD; Kurihara, T; Aguilar, E; Sakimoto, S; Paris, LP; Wittgrove, C; Feitelberg, D; Friedlander, MS; Moreno, SK; Dorrell, MI; Friedlander, M
      The Journal of clinical investigation  125  2335-46  2015

      Mostrar resumen
      25915585 25915585
      Neurite Mistargeting and Inverse Order of Intraretinal Vascular Plexus Formation Precede Subretinal Vascularization in Vldlr Mutant Mice.
      Johnson, V; Xiang, M; Chen, Z; Junge, HJ
      PloS one  10  e0132013  2015

      Mostrar resumen
      26177550 26177550
      Kruppel-Like Factor 4 Regulates Granule Cell Pax6 Expression and Cell Proliferation in Early Cerebellar Development.
      Zhang, P; Ha, T; Larouche, M; Swanson, D; Goldowitz, D
      PloS one  10  e0134390  2015

      Mostrar resumen
      26226504 26226504
      Localization of reelin signaling pathway components in murine midbrain and striatum.
      Sharaf, A; Rahhal, B; Spittau, B; Roussa, E
      Cell and tissue research  359  393-407  2015

      Mostrar resumen
      Mouse25418135 25418135
      Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling.
      Zuckermann, M; Hovestadt, V; Knobbe-Thomsen, CB; Zapatka, M; Northcott, PA; Schramm, K; Belic, J; Jones, DT; Tschida, B; Moriarity, B; Largaespada, D; Roussel, MF; Korshunov, A; Reifenberger, G; Pfister, SM; Lichter, P; Kawauchi, D; Gronych, J
      Nature communications  6  7391  2015

      Mostrar resumen
      26067104 26067104
      Intravenous AAV9 efficiently transduces myenteric neurons in neonate and juvenile mice.
      Gombash, SE; Cowley, CJ; Fitzgerald, JA; Hall, JC; Mueller, C; Christofi, FL; Foust, KD
      Frontiers in molecular neuroscience  7  81  2014

      Mostrar resumen
      Immunofluorescence25360081 25360081
      Mouse model reveals the role of RERE in cerebellar foliation and the migration and maturation of Purkinje cells.
      Kim, BJ; Scott, DA
      PloS one  9  e87518  2014

      Mostrar resumen
      24466353 24466353
      Diversity among principal and GABAergic neurons of the anterior olfactory nucleus.
      Kay, RB; Brunjes, PC
      Frontiers in cellular neuroscience  8  111  2014

      Mostrar resumen
      24808826 24808826
      Detailed expression pattern of aldolase C (Aldoc) in the cerebellum, retina and other areas of the CNS studied in Aldoc-Venus knock-in mice.
      Fujita, H; Aoki, H; Ajioka, I; Yamazaki, M; Abe, M; Oh-Nishi, A; Sakimura, K; Sugihara, I
      PloS one  9  e86679  2014

      Mostrar resumen
      24475166 24475166
      Spontaneous hair cell regeneration in the neonatal mouse cochlea in vivo.
      Cox, BC; Chai, R; Lenoir, A; Liu, Z; Zhang, L; Nguyen, DH; Chalasani, K; Steigelman, KA; Fang, J; Rubel, EW; Cheng, AG; Zuo, J
      Development (Cambridge, England)  141  816-29  2014

      Mostrar resumen
      24496619 24496619

      Ficha técnica

      Cargo
      Anti-Calbindin D-28K - Data Sheet