AB980 Anti-Myelin Basic Protein Antibody

AB980
500 µL  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IHC, IH(P), WB Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB980
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Myelin Basic Protein Antibody
      Alternate Names
      • Myelin A1 protein
      • Myelin membrane encephalitogenic protein
      Background InformationThe classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue, brain lysate
      PresentationLiquid in 0.05 mol/L Tris-HCl, pH 7.6 with 15 mmol/L sodium azide.
      Applications
      ApplicationThis Anti-Myelin Basic Protein Antibody is validated for use in IH, IH(P), WB for the detection of Myelin Basic Protein.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      1:200 dilution of a previous lot was used by PAP or ABC on formalin fixed, paraffin-embedded human spinal cord. Pretreatment of tissue with proteolytic enzyme is not required.

      Western Blot:
      A dilution of 1:3000 was used in TTBS, O/N 4°C on a previous lot.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman myelin basic protein from brain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificitySuitable for labeling of oligodendrocytes and myelin of white matter in brain and spinal cord (gray matter stains less abundantly). Also stains peripheral nerves.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
      Gene Symbol
      • MBP
      • MGC99675
      • Golli-mbp
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation.
      SIZE: 304 amino acids; 33117 Da
      SUBUNIT: Homodimer; isoform 3 exists as a homodimer.
      SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
      TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
      DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter.
      PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
      DISEASE: SwissProt: P02686 # The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
      SIMILARITY: SwissProt: P02686 ## Belongs to the myelin basic protein family.
      Molecular Weight21, 18, 17 & 14 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Mouse brain lysates.

      Western Blot Analysis:
      1:500 dilution of this antibody detected four isoforms of MBP on 10 µg of Mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Myelin Basic Protein Antibody Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-Myelin Basic Protein Antibody Certificados de análisis

      CargoNúmero de lote
      Anti-Myelin Basic Protein - 21369862136986
      Anti-Myelin Basic Protein - 23733102373310
      Anti-Myelin Basic Protein - 23877402387740
      Anti-Myelin Basic Protein - 24284802428480
      Anti-Myelin Basic Protein - 24590122459012
      Anti-Myelin Basic Protein - 20263712026371
      Anti-Myelin Basic Protein - 20539162053916
      Anti-Myelin Basic Protein - 20909152090915
      Anti-Myelin Basic Protein - 21959962195996
      Anti-Myelin Basic Protein - 22193162219316

      Referencias bibliográficas

      Visión general referenciasAplicación Pub Med ID
      The adhesion G protein-coupled receptor GPR56 is a cell-autonomous regulator of oligodendrocyte development.
      Giera, S; Deng, Y; Luo, R; Ackerman, SD; Mogha, A; Monk, KR; Ying, Y; Jeong, SJ; Makinodan, M; Bialas, AR; Chang, BS; Stevens, B; Corfas, G; Piao, X
      Nature communications  6  6121  2015

      Mostrar resumen
      25607655 25607655
      Olig1 function is required for oligodendrocyte differentiation in the mouse brain.
      Dai, J; Bercury, KK; Ahrendsen, JT; Macklin, WB
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  4386-402  2015

      Mostrar resumen
      25762682 25762682
      TREM2 sustains microglial expansion during aging and response to demyelination.
      Poliani, PL; Wang, Y; Fontana, E; Robinette, ML; Yamanishi, Y; Gilfillan, S; Colonna, M
      The Journal of clinical investigation  125  2161-70  2015

      Mostrar resumen
      Immunohistochemistry25893602 25893602
      The pre- and post-somatic segments of the human type I spiral ganglion neurons--structural and functional considerations related to cochlear implantation.
      Liu, W; Edin, F; Atturo, F; Rieger, G; Löwenheim, H; Senn, P; Blumer, M; Schrott-Fischer, A; Rask-Andersen, H; Glueckert, R
      Neuroscience  284  470-82  2015

      Mostrar resumen
      Immunohistochemistry25316409 25316409
      Functionally distinct PI 3-kinase pathways regulate myelination in the peripheral nervous system.
      Heller, BA; Ghidinelli, M; Voelkl, J; Einheber, S; Smith, R; Grund, E; Morahan, G; Chandler, D; Kalaydjieva, L; Giancotti, F; King, RH; Fejes-Toth, AN; Fejes-Toth, G; Feltri, ML; Lang, F; Salzer, JL
      The Journal of cell biology  204  1219-36  2014

      Mostrar resumen
      24687281 24687281
      Coxsackievirus B exits the host cell in shed microvesicles displaying autophagosomal markers.
      Robinson, SM; Tsueng, G; Sin, J; Mangale, V; Rahawi, S; McIntyre, LL; Williams, W; Kha, N; Cruz, C; Hancock, BM; Nguyen, DP; Sayen, MR; Hilton, BJ; Doran, KS; Segall, AM; Wolkowicz, R; Cornell, CT; Whitton, JL; Gottlieb, RA; Feuer, R
      PLoS pathogens  10  e1004045  2014

      Mostrar resumen
      24722773 24722773
      IKAP deficiency in an FD mouse model and in oligodendrocyte precursor cells results in downregulation of genes involved in oligodendrocyte differentiation and myelin formation.
      Cheishvili, D; Dietrich, P; Maayan, C; Even, A; Weil, M; Dragatsis, I; Razin, A
      PloS one  9  e94612  2014

      Mostrar resumen
      24760006 24760006
      Adrenomedullin protects from experimental autoimmune encephalomyelitis at multiple levels.
      Pedreño, M; Morell, M; Robledo, G; Souza-Moreira, L; Forte-Lago, I; Caro, M; O'Valle, F; Ganea, D; Gonzalez-Rey, E
      Brain, behavior, and immunity  37  152-63  2014

      Mostrar resumen
      Immunohistochemistry24321213 24321213
      PDGF is required for remyelination-promoting IgM stimulation of oligodendrocyte progenitor cell proliferation.
      Watzlawik, JO; Warrington, AE; Rodriguez, M
      PloS one  8  e55149  2013

      Mostrar resumen
      23383310 23383310
      Immunophenotyping of inflammatory cells associated with Schmallenberg virus infection of the central nervous system of ruminants.
      Herder, V; Hansmann, F; Wohlsein, P; Peters, M; Varela, M; Palmarini, M; Baumgärtner, W
      PloS one  8  e62939  2013

      Mostrar resumen
      23667545 23667545

      Ficha técnica

      Cargo
      Anti-Myelin Basic Protein - Data Sheet
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies