523121 o-Phenylenediamine, Dihydrochloride, 10-mg Tablets

523121
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          This product has been discontinued.



          We are offering [insert product name] (Cat. No. P8287) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






          Contains 50 tablets. Chromogenic substrate for horseradish peroxidase in ELISA procedures.

          Catalogue Number523121
          Brand Family Calbiochem®
          SynonymsOPD, o-PD, HCl
          References
          Product Information
          CAS number615-28-1
          FormOff-white tablets
          FormulationWhite tablets (10 mg each).
          Hill FormulaC₆H₈N₂ . 2HCl
          Chemical formulaC₆H₈N₂ . 2HCl
          PreservativeNone
          Applications
          Application CommentsThe tablets are inspected visually and are examined spectrophotometrically at 492 nm for the background level in 0.1 M sodium phosphate/citric acid, pH 5.0, containing 6 mM H2O2. Tablets dissolve in the substrate buffer within 5 min (with shaking).

          Suggested Procedure for Use of OPD tablets for HRP-based ELISAs:

          REAGENTS REQUIRED BUT NOT PROVIDED:
          1. Substrate buffer: 50 mM citric acid/sodium phosphate buffer, pH 5.0.
          2. Hydrogen peroxide (H2O2) 30%.
          3. Stop solution: 0.1 M H2SO4.

          PROTOCOL:
          1. Complete all required incubations with antibodies and HRP-labeled probes.
          2. Wash plate wells at least 4 times with phosphate-buffered saline (PBS) or Tris-buffered saline, containing 0.1% TWEEN®-20 detergent.
          3. After the final wash, shake and blot all residual buffer from the wells.
          4. Within 30 min of completion of the above steps, prepare OPD Substrate Solution (the following recipe provides sufficient OPD Substrate Solution for one 96-well plate (100 µl per well):
          a. Remove the OPD tablet container from the refrigerator about 15 min before use.
          b. Dissolve 1 tablet (10 mg total) in 12.5 ml of Substrate Buffer at room temperature with stirring. Note: DO NOT use metal forceps during handling of the tablets, as metals catalyze the oxidation process. Protect the solution from direct sunlight.
          c. Add 12.5 µl of 30% H2O2, mix, and use within 30 min. [Final H2O2 concentration = 0.03% (v/v)]
          5. Add 100 µl of OPD Substrate Solution to each well, and incubate 5-30 min.
          Note: Optimal incubation times may vary depending on the amount of HRP present. If color develops too quickly, dilution of the probe, antibody, or HRP-labeled reagent may be required.
          6. Stop reaction by adding 100 µl of 0.1 M H2SO4.
          7. Read the absorbance at 492 nm.

          Note: Variations in incubation times and reagent concentrations may require further standardization by the user.
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSST0175000
          Safety Information
          R PhraseR: 23/24/25-43-50/53

          Toxic by inhalation, in contact with skin and if swallowed.
          May cause sensitization by skin contact.
          Very toxic to aquatic organisms; may cause long-term adverse effects in the aquatic environment.
          S PhraseS: 28-36/37-45-60-61


          Wear suitable protective clothing and gloves.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          This material and its container must be disposed of as hazardous waste.
          Avoid release to the environment. Refer to special instructions/safety data sheet.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Toxic & Carcinogenic / Teratogenic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsClose the lid of the container tightly for storage, and do not remove the enclosed drying capsule.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          o-Phenylenediamine, Dihydrochloride, 10-mg Tablets Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          o-Phenylenediamine, Dihydrochloride, 10-mg Tablets Certificados de análisis

          CargoNúmero de lote
          523121
          Ficha técnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision10-August-2007 RFH
          SynonymsOPD, o-PD, HCl
          DescriptionOPD (ortho-phenylenediamine) tablets for preparation of soluble substrate.
          BackgroundOPD (ortho-phenylenediamine; o-PD) is a sensitive, chromogenic substrate for horseradish peroxidase (HRP)-based enzyme-linked immunosorbent assays (ELISA). In the presence of HRP and hydrogen peroxide (H2O2), OPD is oxidized to a water-soluble, yellow-orange product that is measured spectrophotometrically at 492 nm, allowing a quantitative analysis of HRP-based systems.
          FormOff-white tablets
          FormulationWhite tablets (10 mg each).
          CAS number615-28-1
          RTECSST0175000
          Chemical formulaC₆H₈N₂ . 2HCl
          PreservativeNone
          CommentsThe tablets are inspected visually and are examined spectrophotometrically at 492 nm for the background level in 0.1 M sodium phosphate/citric acid, pH 5.0, containing 6 mM H2O2. Tablets dissolve in the substrate buffer within 5 min (with shaking).

          Suggested Procedure for Use of OPD tablets for HRP-based ELISAs:

          REAGENTS REQUIRED BUT NOT PROVIDED:
          1. Substrate buffer: 50 mM citric acid/sodium phosphate buffer, pH 5.0.
          2. Hydrogen peroxide (H2O2) 30%.
          3. Stop solution: 0.1 M H2SO4.

          PROTOCOL:
          1. Complete all required incubations with antibodies and HRP-labeled probes.
          2. Wash plate wells at least 4 times with phosphate-buffered saline (PBS) or Tris-buffered saline, containing 0.1% TWEEN®-20 detergent.
          3. After the final wash, shake and blot all residual buffer from the wells.
          4. Within 30 min of completion of the above steps, prepare OPD Substrate Solution (the following recipe provides sufficient OPD Substrate Solution for one 96-well plate (100 µl per well):
          a. Remove the OPD tablet container from the refrigerator about 15 min before use.
          b. Dissolve 1 tablet (10 mg total) in 12.5 ml of Substrate Buffer at room temperature with stirring. Note: DO NOT use metal forceps during handling of the tablets, as metals catalyze the oxidation process. Protect the solution from direct sunlight.
          c. Add 12.5 µl of 30% H2O2, mix, and use within 30 min. [Final H2O2 concentration = 0.03% (v/v)]
          5. Add 100 µl of OPD Substrate Solution to each well, and incubate 5-30 min.
          Note: Optimal incubation times may vary depending on the amount of HRP present. If color develops too quickly, dilution of the probe, antibody, or HRP-labeled reagent may be required.
          6. Stop reaction by adding 100 µl of 0.1 M H2SO4.
          7. Read the absorbance at 492 nm.

          Note: Variations in incubation times and reagent concentrations may require further standardization by the user.
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsClose the lid of the container tightly for storage, and do not remove the enclosed drying capsule.
          Toxicity Toxic & Carcinogenic / Teratogenic
          Merck USA index14, 7284