71327 pET-45b(+) DNA - Novagen

71327
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Elmentette a ()-t
 
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      Árak és elérhetőség megtekintése

      Áttekintés

      Replacement Information

      Árak és elérhetőség

      Katalógusszám ElérhetőségCsomagolás Menny./csomag Ár Mennyiség
      71327-3
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          Muanyagampulla 10 μg
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          Megrendelés befejezésekor További információ
          Elmentette a ()-t
           
          Árajánlat kérése
          Description
          OverviewThe pET-45b plasmid features the ability to express fusion proteins with an N-terminal His•Tag® coding sequence that results in native protein after purification and cleavage. The plasmid contains a strong T7lac promoter, an amino-terminal His•Tag coding sequence, and multiple cloning site (MCS) regions designed to allow the generation of target proteins with minimal vector-encoded fusion. The Pml I cloning site allows direct fusion to the His•Tag sequence for inserts that are blunt and in the appropriate reading frame. For applications requiring a removable amino-terminal His•Tag sequence, the MCS includes a PshA I cloning site (GACNNNNGTC). The PshA I site overlaps the cleavage site for the enterokinase (EK) protease (AspAspAspAspLys). Cloning appropriately designed inserts into this site re-creates the full EK site and allows all amino-terminal vector-encoded sequences to be removed by EK digestion. The remainder of the MCS encodes restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (1).

          The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number71327
          Brand Family Novagen®
          References
          References1. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.
          Product Information
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          pET-45b(+) DNA - Novagen MSDS

          Title

          Safety Data Sheet (SDS) 

          pET-45b(+) DNA - Novagen Certificates of Analysis

          TitleLot Number
          71327

          References

          Hivatkozások áttekintése
          1. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.

          Brochure

          Title
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

          Citations

          Titulus
        • Robert T. Glover, et al. (2007) The two-component regulatory system senX3-regX3 regulates phosphate-dependent gene expression in Mycobacterium smegmatis. Journal of Bacteriology 189, 5495-5503.
        • Guifeng Sun, et al. (2007) Structural and functional analyses of the major outer membrane protein of Chlamydia trachomatis. Journal of Bacteriology 189, 6222-6235.
        • Jun Dai, et al. (2005) The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment. Genes and Development 19, 472-488.
        • User Protocols

          Title
          TB055 pET System Manual

          Vector Map

          Title
          TB396VM pET-45b(+) Vector Map