09-849 Anti-dimethyl MBP (Arg107) Antibody

09-849
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, R WB Rb Serum Polyclonal Antibody
      Description
      Catalogue Number09-849
      DescriptionAnti-dimethyl MBP (Arg107) Antibody
      Alternate Names
      • myelin basic protein
      • Golli-mbp
      • Myelin membrane encephalitogenic protein
      • Myelin A1 protein, MBP
      Background InformationMyelin basic protein (MBP) is the major extrinsic membrane protein of central nervous system. MBP is believed to be important in the process of myelination of nerves in the central nervous system (CNS). MBP was initially sequenced in 1979 after isolation from myelin membranes. Since that time, knockout mice deficient in MBP have been developed which showed decreased amounts of CNS myelination and a progressive disorder characterized by tremors, seizures, and early death. The gene for MBP is on chromosome 18; the protein localizes to the CNS and to various cells of the hematopoietic system. The pool of MBP in the central nervous system is very diverse. Not only is MBP present in a number of splice varients, but the protein also undergoes a variety of post-translational modifications, and exists in the CNS in a number of forms (i.e. phosphorylated, methylated, deamidated and citrullinated).
      Interest in MBP has centered on its role in demyelinating diseases, particularly multiple sclerosis (MS). Several studies have shown a role for antibodies against MBP in the pathogenesis of MS. Some studies have linked a genetic predisposition to MS to the MBP gene, though a majority have not.
      References
      Product Information
      FormatSerum
      Control
      • mouse MBP knockout and wildtype thymus lysates
      PresentationUnpurified rabbit polyclonal in serum containing 0.05% sodium azide.
      Applications
      ApplicationAnti-dimethyl MBP (Arg107) Antibody is an antibody against dimethyl MBP (Arg107) for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of MBP dimethylated at Arg107.
      EpitopeMBP dimethylated at Arg107
      HostRabbit
      SpecificityThis antibody recognizes MBP dimethylated at Arg107.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with mouse. Predicted to react with human and rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq].
      Gene Symbol
      • MBP
      • multiple sclerosis
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation.

      SIZE: 304 amino acids; 33117 Da

      SUBUNIT: Homodimer; isoform 3 exists as a homodimer.

      SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.

      TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter.

      PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.

      DISEASE: The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).

      SIMILARITY: Belongs to the myelin basic protein family.
      Molecular WeightCalculated at 33 kDa, the molecular weight of MBP will vary according to specific splice isoforms and modification states. Dimethylated MBP is observed here at 17, 20 and 28 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse MBP knockout and wildtype thymus lysates.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected dimethylated MBP in 10 µg of thymus lysate from a wildtype mouse , while failing to detect dimethylated MBP in a thymus lysate from an MBP knockout.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-dimethyl MBP (Arg107) Antibody Certificates of Analysis

      TitleLot Number
      Anti-dimethyl MBP (Arg107)2485674
      Anti-dimethyl MBP (Arg107) - NRG1736360NRG1736360

      References

      Reference overviewPub Med ID
      Gait abnormalities and progressive myelin degeneration in a new murine model of Pelizaeus-Merzbacher disease with tandem genomic duplication.
      Clark, K; Sakowski, L; Sperle, K; Banser, L; Landel, CP; Bessert, DA; Skoff, RP; Hobson, GM
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  11788-99  2013

      Show Abstract
      23864668 23864668

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      Life Science Research > Antibodies and Assays > Primary Antibodies