MILLIPLEX® Multiplex Assays Using Luminex®

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Tips and TricksThe Power of Multiplex Biomarker Analysis from the makers of MILLIPLEX® MAP

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Featured Customer Story
Merck:/Freestyle/BI-Bioscience/Protein-Detection/bmia-images/Mukesh-150px.jpgInterested in how MILLIPLEX® MAP assays can further Zika research?

The MILLIPLEX® MAP Rat Cytokine Panel (Cat. No. RECYTMAG-65K) allowed Dr. Mukesh Kumar, of the University of Hawaii, to develop a guinea pig model for Zika virus infection. Learn more about his research by viewing the video below, and read his most recent publication.

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Need Tips on Optimizing your Multiplex Assays?
Merck:/Freestyle/BI-Bioscience/Protein-Detection/bmia-images/Sonali-Nayak-headshot.jpgRead the SelectScience Interview with Sonali Nayak, Bioscience Technical Services Manager, Merck:
Discover How Multiplex Assays are Advancing Biomedical Research: Top tips to optimize your analyses 

Get Sonali Nayak’s take on how to best run your MILLIPLEX® MAP assays.

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As your research uncovers the significant role of more and more biomarkers in both normal and disease states, you need a reliable partner for quality assays and consistent performance. We not only offer you the largest portfolio of biomarker assays—including ELISAs, RIAs and MILLIPLEX® MAP (multi-analyte profiling), our kits and reagents provide you with consistent, high-quality tools so that you can do your best work while saving time, labor and cost.

In addition to the assay specifications listed in the protocol, we evaluate other performance criteria during our validation process: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability).

Each MILLIPLEX® MAP kit includes:
  • Quality controls (QCs) provided to qualify assay performance
  • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
  • Optimized serum matrix to mimic native analyte environment
  • Detection antibody cocktails designed to yield consistent analyte profiles within panel

How to Decide Which MILLIPLEX® Assay Is Best for Your Research

In Method Comparison tests (comparing ELISA and MILLIPLEX® MAP  assays), while the absolute values are not exactly the same, the results do correlate. Hence when switching from one assay method to another, a correction factor may be used when comparing with past data. In most situations the dynamic range and the sensitivity of the assay may be better with the MILLIPLEX® kits.

Please contact Technical Support for more information on correlation factors.

To locate protocols and technical documents for a specific panel, choose any of these options:
  • Search the website for the catalog number.

  • The link to the protocol can be found using:
    • The “Documents” tab
    • The right-hand side of the product name and catalog number
    • The top of the Product Description page
  • To find a panel that contains the analytes you want to measure, use the Analyte Kit Finder at the bottom of this page. Alternatively, type the name of the analyte into the search bar at and use the filters at left to refine your search for “Multiplexing.”

How to Find More Technical Information About Each MILLIPLEX® Assay

  • To find publications citing a specific panel or analyte:
    • Search the website for the catalog number. Links to some of the references using the product can be found at the top of the Product Description Page
    • For a more complete list, contact technical support
  • To determine cross-reactivity for other species for a panel or analyte see the Species Cross-reactivity Tables in our technical guide
  • For intracellular assay kits, we analytically validate the assay only with human cell/tissue culture samples. However, we provide the species homology for each analyte in a table on the product detail page on our website. We have also compiled a list of this information on our website:

    Kits - Kit Species Cross Reactivity

    MAPmate™ Species Cross Reactivity Table

Request the Analyte Quarterly brochure for a complete, updated list of assays.

Find and Price Your Favorite Analytes & Kits –
Two Tools to Make Ordering Faster and Easier!

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MILLIPLEX® Analyte Kit Finder

Use the kit finder below to choose the correct assays for your biomarkers of interest, so you can obtain the best quality data the first time.
Please select the species reactivity:

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Please select your analyte(s) of interest:

Design & Purchase Your Own Kit

Already know your analytes of choice? Start designing and purchasing your own kits now!

Choose Customizable Panels & Premixed Kits – OR – Cell Signaling MAPmates™ Assays.

Design and price your MILLIPLEX® MAP Kits.

Multiplex Customer Testimonial

What are researchers saying about MILLIPLEX® MAP Quality?

“I have used Merck MILLIPLEX® MAP kits for the past four years. Initially I started using these kits to multiplex a specific set of markers, but given their reliability, consistency, and ease of use, I have transitioned all my experiments away from traditional ELISAs. Beyond just being a great product, Merck's customer and technical support is at the top of the industry. The combination of superior product with award-winning support has extended the capabilities of my research, allowing me to answer new novel research questions. I consider MILLIPLEX® MAP kits an important component of my research toolbox.” 

Brian McFarlin, PhD, FACSM, University of North Texas, Denton, Texas

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