152224 AEC

152224
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      Overview

      Replacement Information

      Key Spec Table

      Pricing & Availability

      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      US1152224-10ML
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          Alu drum 10 ml
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          Description
          OverviewPrecipitable peroxidase substrate for immunoblotting and immunohistochemical staining techniques. Produces a red end product that is soluble in alcohol. When used in immunohistochemistry, aqueous mounting media, and counterstains must be used. Directions included for use in each application.
          Catalogue Number152224
          Brand Family Calbiochem®
          Synonyms3-Amino-9-ethylcarbazole, 3AC
          References
          ReferencesOkano, K., et al. 1992. Rinsho Byori 40, 93.
          Krenacs, T., et al. 1990. Histochem. J. 22, 530.
          Product Information
          CAS number132-32-1
          FormYellowish-brown to reddish solution
          Hill FormulaC₁₄H₁₄N₂
          Chemical formulaC₁₄H₁₄N₂
          PreservativeNone
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunohistochemistry
          Application NotesImmunoblotting (see comments)
          Immunohistochemistry (see comments)
          Application CommentsStable at 4°C and 18-26°C. Functionality determined by performing an immunohistologic stain using the final product.

          Suggested procedure for use of AEC in immunohistochemistry and immunoblotting:

          1. Complete all required incubations with antibodies and HRP-labeled probes, and all necessary wash steps.
          2.Prepare 1X AEC Substrate Buffer: Add 9 parts distilled water to 1 part 10X AEC Substrate Buffer, Cat. No. 152226 (0.5 M acetate buffer containing 88 mM H2O2). Mix well. The final pH of this solution will be ~5.0.
          3. Prepare 1X AEC Substrate Solution: Add 1 part 50X AEC Concentrate to 50 parts 1X AEC Substrate Buffer (prepared in Step 2). Mix well.
          4. Following the final wash, completely cover tissue sections with 1X AEC Substrate Solution (prepared in Step 3) and incubate 10-30 min at room temperature. The same conditions apply for immunoblotting. Note: Variables associated with assay conditions will dictate the proper reaction times.
          5. After reaction is complete, wash tissue sections or blotting membranes thoroughly in distilled water.
          6. If tissue sections require a counterstain, Mayer's Hematoxylin followed by blueing in alkaline tap water or a 0.5% aqueous methyl green solution is recommended.
          7. Mount tissue sections in a water-based mounting medium. Air-dry blots and store protected from light.
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSFE3590000
          Safety Information
          R PhraseR: 25

          Toxic if swallowed.
          S PhraseS: 45

          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Toxic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsDiscard if a red or brown precipitate is evident.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Certificates of Analysis

          TitleLot Number
          152224

          References

          Reference overview
          Okano, K., et al. 1992. Rinsho Byori 40, 93.
          Krenacs, T., et al. 1990. Histochem. J. 22, 530.

          Brochure

          Title
          Enzyme-Linked Detection Systems Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-November-2008 JSW
          Synonyms3-Amino-9-ethylcarbazole, 3AC
          ApplicationImmunoblotting (see comments)
          Immunohistochemistry (see comments)
          DescriptionPrecipitable peroxidase substrate for immunoblotting and immunohistochemical staining techniques supplied as a 50X concentrate (0.095 mol/l). To be used with AEC Substrate Buffer, Cat. No. 152226
          BackgroundPrecipitable peroxidase substrate for immunoblotting and immunohistochemical staining techniques. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, AEC is oxidized to a water-insoluble red product. AEC may be used as a substitute for diaminobenzidine (DAB) in HRP-based immunoassays. Although not as sensitive as DAB, the red deposit formed at the sites of AEC oxidation make this substrate useful where double labeling is desired. Since oxidized AEC is soluble in alcohols and xylene, aqueous-based mounting medium and counterstains must be used for immunohistochemical applications.
          FormYellowish-brown to reddish solution
          CAS number132-32-1
          RTECSFE3590000
          Chemical formulaC₁₄H₁₄N₂
          PreservativeNone
          CommentsStable at 4°C and 18-26°C. Functionality determined by performing an immunohistologic stain using the final product.

          Suggested procedure for use of AEC in immunohistochemistry and immunoblotting:

          1. Complete all required incubations with antibodies and HRP-labeled probes, and all necessary wash steps.
          2.Prepare 1X AEC Substrate Buffer: Add 9 parts distilled water to 1 part 10X AEC Substrate Buffer, Cat. No. 152226 (0.5 M acetate buffer containing 88 mM H2O2). Mix well. The final pH of this solution will be ~5.0.
          3. Prepare 1X AEC Substrate Solution: Add 1 part 50X AEC Concentrate to 50 parts 1X AEC Substrate Buffer (prepared in Step 2). Mix well.
          4. Following the final wash, completely cover tissue sections with 1X AEC Substrate Solution (prepared in Step 3) and incubate 10-30 min at room temperature. The same conditions apply for immunoblotting. Note: Variables associated with assay conditions will dictate the proper reaction times.
          5. After reaction is complete, wash tissue sections or blotting membranes thoroughly in distilled water.
          6. If tissue sections require a counterstain, Mayer's Hematoxylin followed by blueing in alkaline tap water or a 0.5% aqueous methyl green solution is recommended.
          7. Mount tissue sections in a water-based mounting medium. Air-dry blots and store protected from light.
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsDiscard if a red or brown precipitate is evident.
          Toxicity Toxic
          ReferencesOkano, K., et al. 1992. Rinsho Byori 40, 93.
          Krenacs, T., et al. 1990. Histochem. J. 22, 530.