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109218 | Gram's crystal violet solution

109218
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      Overview

      Replacement Information

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      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      1092180500
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          Plastic bottle 500 ml
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          1092182500
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              Plastic bottle 2.5 l
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              Description
              Catalogue Number109218
              DescriptionGRAM'S CRYSTAL VIOLET SOLUTION FOR THE G
              OverviewThe Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange. A bottle with 500 ml Gram's crystal violet solution is sufficient for staining up to 250 slides. Gram's crystal violet solution is also available as part of the Gram-color stain set. It is an IVD product and CE registered.
              References
              Product Information
              HS Code3204 19 00
              Applications
              Biological Information
              Physicochemical Information
              Density0.99 g/cm3 (20 °C)
              Flash point47 °C
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Hazard Pictogram(s)
              Hazard Statement(s)H226: Flammable liquid and vapour.
              H412: Harmful to aquatic life with long lasting effects.
              Precautionary Statement(s)P210: Keep away from heat.
              P273: Avoid release to the environment.
              Storage class3 Flammable liquids
              WGKWGK 1 slightly water endangering
              Disposal28
              Aqueous solutions: Container D.
              Safety Information
              Categories of dangerflammable, dangerous for the environment
              R PhraseR 10-52/53
              Flammable.Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
              S PhraseS 61
              Avoid release to the environment. Refer to special instructions/ Safety data sheets.
              Product Usage Statements
              Storage and Shipping Information
              StorageStore at +15°C to +25°C.
              Packaging Information
              Transport Information
              Declaration (railroad and road) ADR, RIDUN 1993 , 3, III
              Declaration (transport by air) IATA-DGRUN 1993 , 3, III
              Declaration (transport by sea) IMDG-CodeUN 1993 , 3, III
              Supplemental Information
              Specifications
              Suitability for microscopypasses test
              Staphylococcus aureusdark blue-violet
              E.colipink to red

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              Alternative Products

              Catalogue Number Description  
              111885 Gram-color Show Pricing & Availability

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              Catalogue Number Description  
              111885 Gram-color Show Pricing & Availability

              Accessories (Primary)

              Catalogue Number Description  
              100567 Lugol's solution Show Pricing & Availability
              109217 Gram's safranine solution Show Pricing & Availability
              109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability
              110218 Gram's decolorizing solution Show Pricing & Availability

              Accessories (Secondary)

              Catalogue Number Description  
              100567 Lugol's solution Show Pricing & Availability
              109217 Gram's safranine solution Show Pricing & Availability
              109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability
              111885 Gram-color Show Pricing & Availability

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              Categories

              IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining