14-786 PI3 Kinase (p110α/p65α) Murine, 10 µg

14-786
10 µg  Also available in 250μg size --call for pricing and availability and reference catalog number 14-786M when ordering the 250μg size.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-786
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPI3 Kinase (p110α/p65α) Murine, 10 µg
      OverviewComplex of N-terminal 6His-tagged, recombinant, full-length mouse p110α and untagged, recombinant, full length mouse p65α.
      Background Informationp65α is an oncogenic mutant form of p85α(residues 1-571) which leads to increased PI3K activity.
      References
      Product Information
      Applications
      ApplicationComplex of N-terminal 6His-tagged, recombinant, full-length mouse p110α & untagged, recombinant, full length mouse p65α. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceCoexpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
      Gene Symbol
      • PIK3CA
      • PI3K
      • MGC142161
      • p110-alpha
      • MGC142163
      Protein TargetPI3Kα/p65α
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyPI3K/PI4K
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
      SIZE: 1068 amino acids; 124284 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
      DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
      SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
      Molecular Weightp110α MW = 129kDa, p65α MW = 65.9kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250μg size --call for pricing and availability and reference catalog number 14-786M when ordering the 250μg size.
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PI3 Kinase (p110α/p65α) Murine, 10 µg SDS

      Title

      Safety Data Sheet (SDS) 

      PI3 Kinase (p110α/p65α) Murine, 10 µg Certificates of Analysis

      TitleLot Number
      PI 3-Kinase (p110α/p65α), murine - 16371851637185

      References

      Reference overviewPub Med ID
      Mechanism of constitutive phosphoinositide 3-kinase activation by oncogenic mutants of the p85 regulatory subunit.
      Shekar, S Chandra, et al.
      J. Biol. Chem., 280: 27850-5 (2005)  2005

      Show Abstract
      15932879 15932879
      Identification and characterization of a new oncogene derived from the regulatory subunit of phosphoinositide 3-kinase.
      Jimenez, C, et al.
      EMBO J., 17: 743-53 (1998)  1998

      Show Abstract
      9450999 9450999

      Technical Info

      Title
      Unlocking the Mystery of Cancer: Do the Mutant p110α Subunits of PI3-Kinase Hold the Key?

      Data Sheet

      Title
      PI 3-Kinase HTRF Assay

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-786M PI3 Kinase (p110α/p65α) Murine, 250 µg Show Pricing & Availability

      Accessories

      Product Families

      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases
      Life Science Research > Proteins and Enzymes > Purified Kinases