ABC32 Anti-Atg4B Antibody

ABC32
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC32
      DescriptionAnti-Atg4B Antibody
      Alternate Names
      • Cysteine protease ATG4B
      • AUT-like 1 cysteine endopeptidase
      • Autophagin-1
      • Autophagy-related cysteine endopeptidase 1
        Autophagy-related protein 4 homolog B
      • hAPG4B
      Background InformationAtg4B (autophagy-related protein 4B) is a member of the Atg4 family of C54 endopeptidases that are required for the synthesis of autophagosomes. Atg4B serves as a cysteine-protease that is essential for LC3 lipidation during autophagosome formation and also for de-lipidation during autophagosome recycling.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Atg4B detects levels of Atg4B proteins & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the human Atg4B.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human.
      Predicted to react with Mouse based on 100% sequence homology. Other homologies: Rat (80% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAutophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
      Gene Symbol
      • ATG4B
      • APG4B
      • AUTL1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

      ENZYME REGULATION: Inhibited by N-ethylmaleimide.

      SUBCELLULAR LOCATION: Cytoplasm.

      TISSUE SPECIFICITY: Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
      Molecular Weight~45 kDa observed.
      5 isoforms are produced by alternative splicing (44 kDa, 52 kDa, 31 kDa, 37 kDa, and 42 kDa). Some unchararterized bands may be observed at ~130 kDa and ~265 kDa in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Atg4B in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Atg4B - 23878532387853
      Anti-Atg4B - NRG1951135NRG1951135

      References

      Reference overviewPub Med ID
      Relocalization of neuronal nitric oxide synthase (nNOS) as a marker for complete restoration of the dystrophin associated protein complex in skeletal muscle.
      Kim E Wells,Silvia Torelli,Qi Lu,Susan C Brown,Terence Partridge,Francesco Muntoni,Dominic J Wells
      Neuromuscular disorders : NMD  13  2003

      Show Abstract
      12467729 12467729

      Newsletters / Publications

      Title
      Autophagy Research Focus