06-863 Anti-CREB Antibody

06-863
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IP, ChIP, ChIP-seq, EMSARbProtein A PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-863
      Replaces04-218
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-CREB Antibody
      Alternate Names
      • Cyclic AMP-responsive element-binding protein 1
      • cAMP-responsive element-binding protein 1
      • CREB-1
      Background InformationCyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation by various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.
      References
      Product Information
      FormatProtein A Purified
      Control
      • HeLa nuclear extract
        Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.
      Applications
      ApplicationAnti-CREB Antibody, Cat. No. 06-863, is a highly specific rabbit Polyclonal antibody, that targets CREB and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      • Electrophoretic Mobility Shift Assay
      Application NotesImmunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated CREB in 500 µg of HeLa nuclear extract.
      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected an enhanced CREB occupancy at the LYPD3 promoter in LKB1-deficient TE-4 human esophageal cancer cells (Gu, Y., et al. (2012). Oncogene. 31(4):469–479).
      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB association at the RNASET2 sites targeted by T-cell Leukemia Virus type 1 (HTLV-1) encoded Tax protein using HTLV-1-infected human T-cell lines, MT-2, C8166/45 and SLB-1 (Polakowski, N., et al. (2011). Viruses. 3(8):1485-500).
      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB occupancy at the hBDNF promoter IV using postmortem human parietal cortex tissue chromatin preparations (Pruunsild, P., et al. (2011). J. Neurosci. 31(9):3295-3308).
      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB occupancy at the miR-9-2 promoter in SK-N-BE human neuroblastoma cells (Laneve, P., et al. (2010). Nucl. Acids Res. 38(20):6895-6905).
      ChIP-seq Analysis: A representative lot identified CREB target genes in murine spermatogonia derived GC1-spg cells by ChIP-seq analysis (Martianov, I., et al. (2010). BMC Genomics. 11:530).
      Western Blotting Analysis: Representative lots detected CREB in lysates from cultured embryonic rat striatal neurons and PC12 pheochromocytoma cells (Hutchinson, A.N., et al. (2012). Neuropsychopharmacology. 37(2):321-337; Maharjan, S., et al. (2010). J. Neurochem. 112(1):42-55).
      Western Blotting Analysis: Representative lots detected CREB in mouse proximal caput epididymis-1 (PC-1) cell lysate and hippocampus tissue homogenates (Hamzeh, M., and Robaire, B. (2011). J. Endocrinol. 209(1):55-64; Zhou, Z., et al. (2009). Neuropharmacology. 56(1):81-89).
      Western Blotting Analysis: A representative lot detected CREB in SK-N-BE human neuroblastoma cell lysate (Laneve, P., et al. (2010). Nucl. Acids Res. 38(20):6895-6905).
      Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of DNA-CREB complex in EMSA using radiolabeled oligonucleotide containing hBDNF promoter I CREB-binding sequence and lysates from KCl-treated primary rat neurons (Pruunsild, P., et al. (2011). J. Neurosci. 31(9):3295-3308).
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (CSGAENQ QSGDAAVTEAENQQ) corresponding to a.a. 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat.
      EpitopeAmino acids 5-24.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes CREB.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Canine, Horse, Porcine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
      Gene Symbol
      • CREB1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16220 # This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE.
      SIZE: 341 amino acids; 36688 Da
      SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain.
      Molecular Weight~43 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected CREB in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-CREB - 21444032144403
      Anti-CREB - 23821312382131
      Anti-CREB - 24468512446851
      Anti-CREB - 1779517795
      Anti-CREB - 2066420664
      Anti-CREB - 22120202212020
      Anti-CREB - 23446542344654
      Anti-CREB - 2358523585
      Anti-CREB - 2536925369
      Anti-CREB - JBC1381972JBC1381972

      References

      Reference overviewApplicationPub Med ID
      Characterization of Aldh2 (-/-) mice as an age-related model of cognitive impairment and Alzheimer's disease.
      D'Souza, Y; Elharram, A; Soon-Shiong, R; Andrew, RD; Bennett, BM
      Molecular brain  8  27  2015

      Show Abstract
      25910195 25910195
      Transcription factor MITF and remodeller BRG1 define chromatin organisation at regulatory elements in melanoma cells.
      Laurette, P; Strub, T; Koludrovic, D; Keime, C; Le Gras, S; Seberg, H; Van Otterloo, E; Imrichova, H; Siddaway, R; Aerts, S; Cornell, RA; Mengus, G; Davidson, I
      eLife  4  2015

      Show Abstract
      25803486 25803486
      MeCP2 phosphorylation limits psychostimulant-induced behavioral and neuronal plasticity.
      Deng, JV; Wan, Y; Wang, X; Cohen, S; Wetsel, WC; Greenberg, ME; Kenny, PJ; Calakos, N; West, AE
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  4519-27  2014

      Show Abstract
      Western Blotting24671997 24671997
      Combinatorial recruitment of CREB, C/EBPβ and c-Jun determines activation of promoters upon keratinocyte differentiation.
      Rozenberg, JM; Bhattacharya, P; Chatterjee, R; Glass, K; Vinson, C
      PloS one  8  e78179  2013

      Show Abstract
      24244291 24244291
      ΔFosB induction in striatal medium spiny neuron subtypes in response to chronic pharmacological, emotional, and optogenetic stimuli.
      Lobo, MK; Zaman, S; Damez-Werno, DM; Koo, JW; Bagot, RC; DiNieri, JA; Nugent, A; Finkel, E; Chaudhury, D; Chandra, R; Riberio, E; Rabkin, J; Mouzon, E; Cachope, R; Cheer, JF; Han, MH; Dietz, DM; Self, DW; Hurd, YL; Vialou, V; Nestler, EJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  18381-95  2013

      Show Abstract
      Immunohistochemistry24259563 24259563
      E2F2 and CREB cooperatively regulate transcriptional activity of cell cycle genes.
      Laresgoiti, U; Apraiz, A; Olea, M; Mitxelena, J; Osinalde, N; Rodriguez, JA; Fullaondo, A; Zubiaga, AM
      Nucleic acids research  41  10185-98  2013

      Show Abstract
      24038359 24038359
      Altered LKB1/CREB-regulated transcription co-activator (CRTC) signaling axis promotes esophageal cancer cell migration and invasion.
      Y Gu,S Lin,J-L Li,H Nakagawa,Z Chen,B Jin,L Tian,D A Ucar,H Shen,J Lu,S N Hochwald,F J Kaye,L Wu
      Oncogene  31  2012

      Show Abstract
      21706049 21706049
      Serum response factor and cAMP response element binding protein are both required for cocaine induction of ΔFosB.
      Vialou, V; Feng, J; Robison, AJ; Ku, SM; Ferguson, D; Scobie, KN; Mazei-Robison, MS; Mouzon, E; Nestler, EJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  7577-84  2012

      Show Abstract
      Western Blotting22649236 22649236
      Differential regulation of MeCP2 phosphorylation in the CNS by dopamine and serotonin.
      Hutchinson, AN; Deng, JV; Aryal, DK; Wetsel, WC; West, AE
      Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology  37  321-37  2012

      Show Abstract
      Western Blotting21956448 21956448
      Distinguishing hyperglycemic changes by Set7 in vascular endothelial cells.
      Okabe, J; Orlowski, C; Balcerczyk, A; Tikellis, C; Thomas, MC; Cooper, ME; El-Osta, A
      Circulation research  110  1067-76  2012

      Show Abstract
      Western Blotting22403242 22403242

      Brochure

      Title
      Western Blotting Tools

      Technical Info

      Title
      Protein Blotting Handbook: 6th Edition

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      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies