07-1007 Anti-IKKα Antibody

07-1007
150 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ca, Eq, B, R, MWB, IPRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1007
      DescriptionAnti-IKKα Antibody
      Alternate Names
      • I kappa-B kinase alpha
      • I-kappa-B kinase 1
      • I-kappa-B kinase-alpha
      • IKK-a kinase
      • IkB kinase alpha subunit
      • IkappaB kinase
      • Nuclear factor NF-kappa-B inhibitor kinase alpha
      • Nuclear factor NFkappaB inhibitor kinase alpha
      • conserved helix-loop ubiquitous kinase
      • conserved helix-loop-helix ubiquitous kinase
      Background InformationThe TNF and IL-1 signaling pathways activate the IκB kinases, IKKα (IKK1), IKKβ (IKK2). Phosphorylation of the IκB cytoplasmic inhibitors, IκBα, IκBβ and IκBγ by these kinases triggers proteolytic degradation and the release of NFκB/Rel proteins from the IκB inhibitors. This allows the NFκB proteins to enter the nucleus. A third member of the family, IKKε (IKKI), is not induced by TNF or IL-1. IKKε together with TANK-binding kinase-1 (TBK1), is essential for resistance to viral infection; either kinase can phosphorylate IRF3 and IRF7, leading to their nuclear translocation and induction of interferon and ISG56 gene expression.
      References
      Product Information
      FormatPurified
      Control
      • MCF-7 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.
      Applications
      ApplicationAnti-IKKα Antibody detects level of IKKα & has been published & validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 5-20 µg from a previous lot was used in IP.
      Western Blot Analysis (SNAP ID Technique): 1 µg/mL from a previous lot detected IKKα in 10 µg of MCF-7 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to IKKα.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes IKKα.
      Species Reactivity
      • Human
      • Canine
      • Horse
      • Bovine
      • Rat
      • Mouse
      Species Reactivity NoteProven to react with human. Predicted to react with rat, mouse, equine (horse), canine (dog), and bovine (cow) based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
      Gene Symbol
      • EC 2.7.11.10
      • IKBKA
      • IKK-A
      • IKK-alpha
      • IKK1
      • IKKA
      • IkBKA
      • NFKBIKA
      • OTTHUMP00000020273
      • TCF16
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
      CATALYTIC ACTIVITY: ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].
      ENZYME REGULATIION: Activated when phosphorylated and inactivated when dephosphorylated.
      SUBUNIT STRUCTURE: Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Directly interacts with IKK-gamma/NEMO and TRPC4AP By similarity. May interact with TRAF2. Interacts with NALP2. May interact with MAVS/IPS1.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and the nucleus.
      TISSUE SPECIFICITY: Widely expressed.
      PTM: Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
      Molecular Weight~80-85 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in MCF-7 cell lysate.
      Western Blot Analysis: 1-4 µg/mL of this antibody detected IKKα in 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size150 µg
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      05-536 Anti-IKKα Antibody, clone 14A231 Show Pricing & Availability
      ABS812 Anti-IKK alpha Antibody Show Pricing & Availability
      MABE628 Anti-CHUK, clone 3G12H9 Antibody Show Pricing & Availability
      MABF222 Anti-IKKa (p45) Antibody, clone 881H3 Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies