07-405 Anti-PRMT5 Antibody

07-405
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-405
      ReplacesMABE551
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PRMT5 Antibody
      OverviewPRMT5 (aka JBP1 Janus Binding Protein1) is a member of a family of enzymes that deposit methyl groups on arginine residues of proteins, including both histone and non-histone substrates.
      Alternate Names
      • 72 kDa ICln-binding protein
      • HMT1 hnRNP methyltransferase-like 5
      • Jak-binding protein 1
      • SKB1 homolog
      • SKB1 homolog (S. pombe)
      • Shk1 kinase-binding protein 1 homolog
      • protein arginine methyltransferase 5
      • skb1 (S. pombe) homolog
      Background InformationArginine methylation of proteins is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT5 (also known as Skb1Hs/JBP1) exists as homooligomeric complexes, which includes a dimer and tetramer. Homooligomerization of PRMT5 has an important role in its ability to methylate the MBP protein.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate, HeLa cell lysate.

        Included Positive Antigen Control:
        Catalog # 12-303, Jurkat lysate. Add 2.5 μL of 2- mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified rabbit IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.
      Applications
      ApplicationAnti-PRMT5 Antibody is a Rabbit Polyclonal Antibody for detection of PRMT5 also known as 72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog & has been validated in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      An independent laboratory has reported that this antibody immunoprecipitates active PRMT5 from HeLa cell lysate.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 60-79 (KNRPGPQTRSDLLLSGRDWN) of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityPRMT5, Mr ~70 kDa
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRMT5
      • HRMT1L5
      • IBP72
      • SKB1Hs
      • JBP1
      • SKB1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O14744 # Methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A/H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription.
      SIZE: 637 amino acids; 72684 Da
      SUBUNIT: Forms, at least, homodimers and homotetramers. Interacts with PRDM1 (By similarity). Component of the methylosome, a 20S complex containing at least pICLn, PRMT1/SKB1 and MEP50. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SUPT5H. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Ubiquitous.
      PTM: Disulfide bonds and non-covalent association mediate homooligomers formation.
      SIMILARITY:SwissProt: O14744 ## Belongs to the protein arginine N-methyltransferase family.
      Molecular Weight~70 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot in RIPA lysates from 3T3/A31, NIH 3T3,HeLa, WI-38, and Jurkat cells.

      Western Blot Analysis:
      0.5-1 μg/mL of this lot detected PRMT5 in RIPA lysates from 3T3/A31, NIH 3T3, HeLa, WI-38, and Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PRMT52484181
      Anti-PRMT5 (rabbit polyclonal IgG) - DAM1412892DAM1412892
      Anti-PRMT5 - 24247692424769
      Anti-PRMT5 - 24501762450176
      Anti-PRMT5 - 19939431993943
      Anti-PRMT5 - 20809182080918
      Anti-PRMT5 - 22025462202546
      Anti-PRMT5 - 23251132325113
      Anti-PRMT5 - 2433424334
      Anti-PRMT5 - 3262632626

      References

      Reference overviewApplicationPub Med ID
      SOX17 is a critical specifier of human primordial germ cell fate.
      Irie, N; Weinberger, L; Tang, WW; Kobayashi, T; Viukov, S; Manor, YS; Dietmann, S; Hanna, JH; Surani, MA
      Cell  160  253-68  2015

      Show Abstract
      25543152 25543152
      Prmt5 is required for germ cell survival during spermatogenesis in mice.
      Wang, Y; Zhu, T; Li, Q; Liu, C; Han, F; Chen, M; Zhang, L; Cui, X; Qin, Y; Bao, S; Gao, F
      Scientific reports  5  11031  2015

      Show Abstract
      26072710 26072710
      Interplay between arginine methylation and ubiquitylation regulates KLF4-mediated genome stability and carcinogenesis.
      Hu, D; Gur, M; Zhou, Z; Gamper, A; Hung, MC; Fujita, N; Lan, L; Bahar, I; Wan, Y
      Nature communications  6  8419  2015

      Show Abstract
      26420673 26420673
      PRMT5 is required for human embryonic stem cell proliferation but not pluripotency.
      Gkountela, S; Li, Z; Chin, CJ; Lee, SA; Clark, AT
      Stem cell reviews  10  230-9  2014

      Show Abstract
      24477620 24477620
      The SWI/SNF subunit/tumor suppressor BAF47/INI1 is essential in cell cycle arrest upon skeletal muscle terminal differentiation.
      Joliot, V; Ait-Mohamed, O; Battisti, V; Pontis, J; Philipot, O; Robin, P; Ito, H; Ait-Si-Ali, S
      PloS one  9  e108858  2014

      Show Abstract
      Western Blotting25271443 25271443
      Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune response genes.
      Tsutsui, T; Fukasawa, R; Shinmyouzu, K; Nakagawa, R; Tobe, K; Tanaka, A; Ohkuma, Y
      The Journal of biological chemistry  288  20955-65  2013

      Show Abstract
      23749998 23749998
      Alternative splicing regulates Prdm1/Blimp-1 DNA binding activities and corepressor interactions.
      Morgan, MA; Mould, AW; Li, L; Robertson, EJ; Bikoff, EK
      Molecular and cellular biology  32  3403-13  2012

      Show Abstract
      22733990 22733990
      Epigenetic mechanisms of Groucho/Grg/TLE mediated transcriptional repression.
      Patel, SR; Bhumbra, SS; Paknikar, RS; Dressler, GR
      Molecular cell  45  185-95  2012

      Show Abstract
      22169276 22169276
      Role of type II protein arginine methyltransferase 5 in the regulation of Circadian Per1 gene.
      Na, J; Lee, K; Kim, HG; Shin, JY; Na, W; Jeong, H; Lee, JW; Cho, S; Kim, WS; Ju, BG
      PloS one  7  e48152  2012

      Show Abstract
      23133559 23133559
      Arginine Methyltransferases Are Regulated by Epstein-Barr Virus in B Cells and Are Differentially Expressed in Hodgkin's Lymphoma.
      Leonard, S; Gordon, N; Smith, N; Rowe, M; Murray, PG; Woodman, CB
      Pathogens (Basel, Switzerland)  1  52-64  2012

      Show Abstract
      25436604 25436604

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      Included Positive Control

      Catalogue Number Description  
      12-303 Jurkat Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies