SCC064 LX-2 Human Hepatic Stellate Cell Line

SCC064
≥1X10⁶ cells/vial   
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCC064
      DescriptionLX-2 Human Hepatic Stellate Cell Line
      Alternate Names
      • HSC
      • Perisinusoidal cells
      • Ito cells
      • Hepatic lipocytes
      • Hepatic pericytes
      Background InformationHepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.

      LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.

      Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.
      References
      Product Information
      Components
      • 1) ≥1X106 viable LX-2 Human Hepatic Stellate Cells: (Catalog No. SCC064). Store in liquid nitrogen.
      Applications
      ApplicationThe LX-2 human hepatic stellate cell line has been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis , making them a highly suitable model of human hepatic fibrosis.
      Key Applications
      • Stem Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Hepatocyte Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested by PCR and are negative for Hepatitis A, B, C and HIV-1 & 2 viruses.
      • Cells are negatrive for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsLX-2 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality. LX-2 cells have been successfully expanded past passage 50 in culture.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Hepatitis C virus core protein stimulates fibrogenesis in hepatic stellate cells involving the obese receptor.
      Wu, Ching-Fen, et al.
      J. Cell. Biochem., 114: 541-50 (2013)  2013

      Show Abstract
      22961938 22961938
      Expression of Septin4 in human hepatic stellate cells LX-2 stimulated by LPS.
      Sun, Xiaolei, et al.
      Inflammation, 36: 539-48 (2013)  2013

      Show Abstract
      23180367 23180367
      TRAIL regulates collagen production through HSF1-dependent Hsp47 expression in activated hepatic stellate cells.
      Park, Soo-Jung, et al.
      Cell. Signal., 25: 1635-43 (2013)  2013

      Show Abstract
      23587601 23587601
      Clinical significance and gene expression study of human hepatic stellate cells in HBV related-hepatocellular carcinoma.
      Liao, Rui, et al.
      J. Exp. Clin. Cancer Res., 32: 22 (2013)  2013

      Show Abstract
      23601182 23601182
      Interleukin-21 mediates hepatitis B virus-associated liver cirrhosis by activating hepatic stellate cells.
      Feng, Guohua, et al.
      Hepatol. Res., (2013)  2013

      Show Abstract
      23905760 23905760
      Brucella abortus induces collagen deposition and MMP-9 down-modulation in hepatic stellate cells via TGF-β1 production.
      Arriola Benitez, Paula C, et al.
      Am. J. Pathol., 183: 1918-27 (2013)  2013

      Show Abstract
      24113459 24113459
      Genetic characteristics of the human hepatic stellate cell line LX-2.
      Weiskirchen, Ralf, et al.
      PLoS ONE, 8: e75692 (2013)  2013

      Show Abstract
      24116068 24116068
      Epigenetic regulation of connective tissue growth factor by microRNA-214 delivery in exosomes from mouse or human hepatic stellate cells.
      Chen, Li, et al.
      Hepatology, (2013)  2013

      Show Abstract
      24122827 24122827
      Human hepatic stellate cell lines, LX-1 and LX-2: new tools for analysis of hepatic fibrosis.
      Xu, L, et al.
      Gut, 54: 142-51 (2005)  2005

      Show Abstract
      15591520 15591520
      Isolated hepatic lipocytes and Kupffer cells from normal human liver: morphological and functional characteristics in primary culture.
      Friedman, S L, et al.
      Hepatology, 15: 234-43 (1992)  1992

      Show Abstract
      1735526 1735526

      Brochure

      Title
      New Products - Antibodies, Assays, Small Molecules, Inhibitors, and Proteins Volume 2, 2014

      User Guides

      Title
      LX-2 Human Hepatic Stellate Cell Line