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A Flow Cytometry System with Patented Optics and a Highly Configurable
The CellStream™ benchtop flow cytometer features patented Amnis® optics camera technology and the ability to configure the system with 1 to 7 lasers. This gives researchers unparalleled sensitivity plus flexibility to customize and expand the system according to research needs and budget.
Compatible with Many Common Fluorochromes and Fluorescent Reagents
Thoughtful laser and detector selection ensures that CellStream™ instruments are compatible with familiar and trusted fluorophores, as well as many of the newer dyes that offer advantages such as increased brightness or photo-stability.
CellStream™ System Architecture
Our patented time delay integration (TDI) and camera technology delivers sensitivity and expandability beyond what is possible with traditional flow cytometers.
- Up to 7 lasers are focused in discrete locations.
- Hydrodynamically focused cells pass through the laser-illuminated region. Fluorochromes bound to the cells are excited and emit into the collection system. Fluorescence is collected and directed toward an intermediate image plane.
- The filter stack decomposes each of the four discrete vertical positions in the intermediate image plane into 22 separate channels of data.
- All 22 channels fit efficiently onto a CCD (charge-coupled device) array.
CellStream™ system’s sensor contains multiple discrete collection fields using the same CCD (charge-coupled device) as patented Amnis® technology.
How CellStream™ Flow Cytometry Optics Work
Charge-Coupled Device (CCD) Camera in Time-Delay Integration
CellStream™ systems, like our state-of-the-art Amnis® flow cytometers, employ a time-delayed integration (TDI), charge-coupled device (CCD) camera. This is the same technology used in medical radiography and military applications to capture images of moving objects.
The custom camera within CellStream™ flow cytometers operates using this TDI technique, whereby a specialized detector readout mode preserves sensitivity and image quality, even with fast relative movement between the detector and the objects being imaged. The TDI detection technology of the CCD camera allows up to 1000 times more signal to be acquired from cells in flow than from conventional frame imaging approaches. Velocity detection and autofocus systems maintain proper camera synchronization and focus during the process of image acquisition.
As with any CCD, image photons are converted to photocharges in an array of pixels. During TDI operation, the photocharges are continuously shifted from pixel to pixel down the detector, parallel to the axis of flow, integrating the signal over multiple pixels. By synchronizing the photocharge shift rate with the velocity of the flowing cell, the effect is similar to physically panning a camera. With TDI, image streaking is avoided despite signal integration times that are orders of magnitude longer than those of conventional flow cytometry. Each pixel is digitized with 12 bits of intensity resolution, providing a minimum dynamic range of three decades per pixel.