This high level course explores the implementation of cutting-edge tangential flow filtration (TFF) techniques and technologies. This course includes a choice of technically rigorous modules which address high-level scientific concerns within tangential flow filtration design and implementation, including high-viscosity tangential flow filtration (HVTFF), single-pass tangential flow filtration design (SPTFF) and operation, and two-pump microfiltration (MFTFF).
There is an increasing need for high concentration biopharmaceuticals. Tangential flow filtration is typically used to concentrate the drug substance, and this module addresses the many challenges associated with conducting very high-concentration endpoint tangential flow filtration operations. This module evaluates why high-concentration tangential flow filtration processes are difficult to implement and how these challenges can be surmounted during process development.
Single-pass tangential flow filtration has been proposed as an effective unit operation for concentration of product either continuously or for products where multiple pump passes are unacceptable. This module addresses the background and capabilities of SPTFF together with implementation strategies.
Microporous and “open UF” tangential flow filtration operations are commonly used for primary or secondary clarification of products, such as lysates and cell cultures. These highly permeable membranes can lead to very high initial conversions but poor fluxes over the length of the process. This module investigates why this phenomenon occurs and provides novel system design strategies to prevent it.
The courses are modular and can be selected individually or in combination with each other.
The laboratory exercise takes place using a realistic feed stream and typical process conditions. Depending on the advanced modules chosen, the laboratory sessions may include: