Oxidative Stress Assays
OxyBlot™ Protein Oxidation Detection KitThe OxyBlot™ Protein Oxidation Detection Kit provides the chemical and immunological reagents necessary to perform the immunoblot detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation.
Sensitivity of DNP Detection Using the OxyBlot™ Kit.
DNP in serial dilutions of DNP-protein mixture provided in the kit were analyzed as described in the protocol. Exposure time was 1 minute. DNP in BSA Band: lane 1: 100 femtomoles, lane 2: 30 femtomoles, lane 3: 10 femtomoles, lane 4: 3 femtomoles, lane 5: 1 femtomoles, lane 6: 0.3 femtomoles, lane 7: 0.1 femtomoles
OxyICC™ Oxidized Protein Detection KitThe OxyICC™ Oxidized Protein Detection Kit provides reagents for fluorescent immunocytochemical detection of cellular protein carbonyls. This simple assay detects carbonyl modifications using dinitrophenylhydrazine (DNPH) to provide highly sensitive and quantitative results.