Simplicon™ RNA Reprogramming Technology

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Single Transfection. Virus-Free iPSCs.

The Simplicon™ RNA Reprogramming Technology utilizes a single, polycistronic, synthetic, self-replicating RNA strand that is sufficient for generating high numbers of human iPSCs with a single transfection step. The single RNA contains the four reprogramming factors, OCT-4, KLF-4, SOX-2 and GLIS1, and allows for efficient reprogramming without viral intermediates or host genome integration.

With Simplicon™ RNA Reprogramming Technology, you can:
  • Create integration-free iPS cells using synthetic RNA
  • Reprogram efficiently and easily using a single transfection step
  • Achieve controlled elimination of reprogramming factors

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New Article - Simplicon™ In Vivo Protein Expression System: A non-genome integrating, tunable and sustained protein expression system in human cells

Technology Featured in Cell Stem Cell:

Yoshioka, Naohisa, et al. Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell 13.2 (2013): 246-254.

Why Reprogram with Synthetic RNA?
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Typical Results with Single Transfection – Highly Efficient
Merck:/Freestyle/BI-Bioscience/Cell-Culture/stem-cell-images/hff-fibroblast.jpg Merck:/Freestyle/BI-Bioscience/Cell-Culture/stem-cell-images/bj-fibroblast.jpg
HFF Fibroblast BJ fibroblast

Alkaline phosphatase staining of reprogrammed fibroblasts generated using Simplicon™ RNA Reprogramming Technology.

Human iPS cells, generated using Simplicon™ self-replicating RNA (OKSG), express pluripotency markers Oct-4, Sox-2, TRA-1-60 and Nanog 21 days after initial transfection.
Human iPS cells, generated using Simplicon™ self-replicating RNA (OKSG), express pluripotency markers Oct-4, Sox-2, TRA-1-60 and Nanog 21 days after initial transfection.