Primary Mouse Embryonic Fibroblasts

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MEF Feeder Cells for ES Cell Culture

PMEF feeder cells at the correct density
Many embryonic stem (ES) cell culture protocols use a monolayer of primary mouse embryo fibroblast (PMEF) feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Derived from day 13 embryos, these cells are frozen into individual vials, containing approximately 5-6 x 106 fibroblasts per vial, and are sold as a pack of 5 vials per kit at various passage number (P1-P3). Several varieties are available, including drug resistant, growth arrested, and not mitomycin-C treated feeder cells.

PMEF-CF1:
PMEF cells isolated from CF-1 mouse embryos at day 13. Most commonly used PMEF cell line used to support mouse and human embryonic stem cells in culture.

PMEF-N:
Neomycin resistant PMEF cells isolated from FVB mouse embryos at day 13. Commonly used in genetic selection experiments using neomycin as selection agent.

PMEF-H: 
Hygromycin resistant PMEF cells isolated from C57BL/6 mouse embryos at day 13.

PMEF-DR4: 
DR4 MEF feeder cells are resistant to neomycin, hygromycin, puromycin, and 6-thioguanine.

PRODUCT DESCRIPTIONTREATMENTPASSAGEQTY/PKCAT. NO.
EmbryoMax® Primary Mouse
Embryo Fibroblasts
Strain CF1
Not Treated P1 1 vial, 1 x 106 cells ea. PMEF-CFL-P1
Not Treated P3 5 vials, 5-6 x 106 cells ea. PMEF-CFL
Mitomycin-C Treated P3 5 vials, 5-6 x 106 cells ea. PMEF-CF
Irradiated P3 5 vials, 5-6 x 106 cells ea. PMEF-CFX
EmbryoMax® Primary
Mouse Embryo Fibroblasts
Neo Resistant
Not Treated P1 1 vial, 1 x 106 cells ea. PMEF-NL-P1
Not Treated P3 5 vials, 5-6 x 106 cells ea. PMEF-NL
Mitomycin-C Treated P3 5 vials, 5-6 x 106 cells ea. PMEF-N
Irradiated P3 5 vials, 5-6 x 106 cells ea. PMEF-NX
EmbryoMax® Primary Mouse
Embryo Fibroblasts
Hygro Resistant
Not Treated P3 5 vials, 5-6 x 106 cells ea. PMEF-HL
Mitomycin-C Treated P3 5 vials, 5-6 x 106 cells ea. PMEF-H
EmbryoMax® Primary Mouse
Embryo Fibroblasts
Strain DR4
Mitomycin-C Treated P3 1 vials, 4 x 106 cells ea. PMEF-DR4
Mitomycin-C Treated P3 5 vials, 4 x 106 cells ea. PMEF-DR4-5P
Irradiated P3 1 vials, 4 x 106 cells ea. PMEF-DR4X
Irradiated P3 5 vials, 4 x 106 cells ea. PMEF-DR4X-5P

Interested in purchasing an entire lot of PMEF’s? Bulk sized lots of PMEF are available with lot sampling.