Overnight Express™ Autoinduction Systems

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Merck:/Freestyle/BI-Bioscience/Genomic-Analysis/overnight-express-cover.jpgOvernight Express™ Autoinduction Systems
The protein expression process begins without having to manually add induction agent. We've done it for you!

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth. The simplified protocol offers great convenience, allowing you to focus on your research while it does its job.

Below we've broken out the best products for each usage. Use this to select the correct product for your application.

Most Routine Recombinant Protein Expression Applications

Complete autoinduction media in granulated Luria-Bertani formulation
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Overnight Express™ Instant LB Medium
Complete autoinduction media in granulated Terrific Broth formulation
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Overnight Express™ Instant TB Medium
Autoinduction media to be added to glucose-free medium (e.g., 2X YT, SOC, LB and TB)
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Overnight Express™ Autoinduction System 1


For X-Ray Crystallography

Compatible with selenomethionyl (Se-Met) labeling of proteins
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Overnight Express™ Autoinduction System 2


For NMR Spectroscopy

Determine optimal culture conditions for high-level protein expression before isotopic protein labeling. It can also be used for 15N protein labeling when user provides 15N-ammonium chloride.
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Overnight Express™ NMR Medium - Optimization
High level incorporation of 15N for initial NMR analysis to assess suitability for structure determination
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Overnight Express™ NMR Medium - 15N
High level incorporation of 15N and 13C for backbone and side-chain assignments and for restraint measurements in structure determination
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Overnight Express™ NRM Medium - 15N, 13C