Single Molecule Counting (SMC™) Technology Platform

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Accelerating Drug Development & Clinical Validation with Single Molecule Counting

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At femtograms per mL, it was undetectable…
Learn why Dr. Erika Cline switched to Singulex Erenna® assays.

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Unleashing the Sensitivity of Single Molecule Counting (SMC™) Technology

Ultrasensitive SMC™ technology, originally developed by Singulex, Inc., provides an indispensable tool in the researcher’s arsenal to help them move novel biology forward, fueling the discovery and development of new therapeutics.

Merck now offers the power of SMC™ technology to leading pharmaceutical R&D laboratories, clinical research organizations (CROs) and academic institutions around the world through the Research-Use-Only Erenna® Immunoassay System, immunoassay kits, reagents, and custom services.

Singulex Erenna® Instrument
SMC™ Erenna® Instrument with SMC™ technology quantifies low-abundance biomarkers, on the bench top.

Previously Undetectable, Now Quantified. One Molecule at a Time.

SMC™ Single Molecule Counting (SMC™) technology enables precise measurement of molecules at levels previously undetectable, for unprecedented biomarker insights.

Scientists familiar with traditional immunoassays, such as ELISAs, know that sensitivity, matrix effects, and dynamic range affect how they make measurements—or if they can make them at all.

Combining a traditional immunoassay workflow with patented SMC™ technology enables the detection of low-abundance biomarkers, such as proteins and nucleic acids, with unparalleled sensitivity and accuracy, capturing concentrations down to the femtogram/mL level. Researchers can now detect, and monitor changes in, extremely low levels of established disease biomarkers such as cardiac troponin I and cytokines.

What Can Better Precision and Sensitivity Do for Your Research?

  • Quantify previously undetectable analytes
  • Better stratify sample populations
  • Gain insights into novel biological mechanisms
  • Require fewer data points for critical decision making
  • Accelerate drug discovery and development
  • Reduce program costs and improve productivity

Click here to download our latest Singulex Erenna® immunoassay kits, Custom Services, and the Erenna® Immunoassay System brochure.
SMC™ Assay Workflow. During the capture and detection steps, specific antibodies translate each biomarker into a signal. During the modified elution step, fluorescent dye-labeled detection antibodies are released from the immune complexes. The eluate is then drawn into the Erenna® System capillary tube, which contains a very small interrogation space that is illuminated by a laser. Single fluorescently labeled molecules are detected as they generate intense flashes of light when passing through the interrogation space. Detected signals with peak intensity above the threshold of background fluorescence are counted as digital events.