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209550 Anti-Cannabinoid Receptor CB₁ (1-77) Rabbit pAb

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209550
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      Ch, F, Gr, H, Mk, M, R, SalRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~60 kDa CB1 receptor protein.
      Catalogue Number209550
      Brand Family Calbiochem®
      Application Data
      Detection of rat cannabinoid receptor 1 by immunohistochemistry. Sample: Rat hippocampus tissue. Primary antibody: Anti-Cannabinoid Receptor CB1 (1-77) Rabbit pAb (Cat. No. 209550) (1:1000). Detection: fluorescence.

      Detection of chicken cannabinoid receptor 1 by immunohistochemistry. Sample: Transverse spinal cord sections of E6 chick embryo. Primary antibody: Anti-Cannabinoid Receptor CB1 (1-77) Rabbit pAb (Cat. No. 209550) (Dilution Assay Dependent). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
      Positive controlHippocampal neurons
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesSpecies Cross-Reactivity

      Schomacher, M., et al. 2006. Acta Neuropathol. 111, 8.
      Small-Howard, A.L., et al. 2005. Biochem. J. 388, 465.
      Bouchard, J.F., 2003. Life Sci. 72, 1859.
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesImmunoblotting (1:1000)
      Immunocytochemistry (1:100-1:1000)
      Paraffin Sections (see comments)
      Application CommentsMay also recognize weaker bands at ~23 kDa, ~72 kDa, and ~180 kDa in some tissues. Immunocytochemical staining of living or fixed cultured hippocampal neurons show intense, punctate staining along processes with little staining over cell bodies. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena recombinant protein consisting of the first 77 amino acids of rat CB₁ receptor
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Fish
      • Gerbil
      • Human
      • Monkey
      • Mouse
      • Rat
      • Salamander
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      209550 0

      Documentation

      Anti-Cannabinoid Receptor CB₁ (1-77) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cannabinoid Receptor CB₁ (1-77) Rabbit pAb Certificates of Analysis

      TitleLot Number
      209550

      Citations

      Title
    • Bouchard, J.F., 2003. Life Sci. 72, 1859.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-October-2010 RFH
      ApplicationImmunoblotting (1:1000)
      Immunocytochemistry (1:100-1:1000)
      Paraffin Sections (see comments)
      Application Data
      Detection of rat cannabinoid receptor 1 by immunohistochemistry. Sample: Rat hippocampus tissue. Primary antibody: Anti-Cannabinoid Receptor CB1 (1-77) Rabbit pAb (Cat. No. 209550) (1:1000). Detection: fluorescence.

      Detection of chicken cannabinoid receptor 1 by immunohistochemistry. Sample: Transverse spinal cord sections of E6 chick embryo. Primary antibody: Anti-Cannabinoid Receptor CB1 (1-77) Rabbit pAb (Cat. No. 209550) (Dilution Assay Dependent). Detection: fluorescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa CB1 receptor protein.
      BackgroundCannabinoids exert their physiological effects through two G-protein coupled receptors, CB1 and CB2. Both receptors inhibit adenylyl cyclase and stimulate MAP kinase. CB1 receptors also modulate ion channels through direct G protein interactions. D-9-tetrahydrocannibinol and related ligands exert their psychoactive effects by inhibiting presynaptic N- and P/Q-type calcium channels. CB1 receptors are primarily expressed in brain (particularly cortex, striatum, hippocampus and cerebellum), spinal cord, eye, and testis. CB1 receptors are involved in the modulation of nociception, movement, learning and memory, emotion, and many other neuronal processes.
      HostRabbit
      Immunogen speciesRat
      Immunogena recombinant protein consisting of the first 77 amino acids of rat CB₁ receptor
      IsotypeIgG
      Specieschicken, fish, gerbil, human, monkey, mouse, rat, salamander
      Positive controlHippocampal neurons
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
      Preservative≤0.1% sodium azide
      CommentsMay also recognize weaker bands at ~23 kDa, ~72 kDa, and ~180 kDa in some tissues. Immunocytochemical staining of living or fixed cultured hippocampal neurons show intense, punctate staining along processes with little staining over cell bodies. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      Citation
    • Bouchard, J.F., 2003. Life Sci. 72, 1859.
      		•
      Application referencesSpecies Cross-Reactivity

      Schomacher, M., et al. 2006. Acta Neuropathol. 111, 8.
      Small-Howard, A.L., et al. 2005. Biochem. J. 388, 465.
      Bouchard, J.F., 2003. Life Sci. 72, 1859.