516675 | 4EBP1/PHAS-I, Rat, Recombinant

516675
  
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      Overview

      Replacement Information
      Description
      OverviewA 117-amino acid protein substrate which has been shown to be an excellent substrate for MAP kinase, p38 kinase, PKC, and JNK. Phosphorylation of PHAS-I increases in response to insulin but not to cAMP stimulation of adipocytes. An excellent substrate for MAP kinase both in vivo and in vitro. Reported to be a substrate for mammalian and yeast TOR phosphorylation.
      Catalogue Number516675
      Brand Family Calbiochem®
      SynonymsPhosphorylated Heat- and Acid-stable Protein Regulated by Insulin
      References
      ReferencesAlarcon, C.M., et al. 1999. Mol. Biol. Cell 10,2531. Hu, C., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3730. Pause, A., et al. 1994. Nature 371, 762. Brunn, G.J., et al. 1991. Science 277, 99.
      Product Information
      FormLiquid
      FormulationIn 50 mM NaCl, 25 mM Tris-HCl, 5 mM β-mercaptoethanol, 500 µM EDTA, pH 7.4.
      Applications
      Biological Information
      Biological activityShown to undergo phosphorylation by activated MAP kinase (Cat. No. 454855) in the presence of ³²P-ATP.
      Purity≥95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      516675

      References

      Reference overview
      Alarcon, C.M., et al. 1999. Mol. Biol. Cell 10,2531. Hu, C., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3730. Pause, A., et al. 1994. Nature 371, 762. Brunn, G.J., et al. 1991. Science 277, 99.

      Brochure

      Title
      MAP Kinases Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-July-2007 RFH
      SynonymsPhosphorylated Heat- and Acid-stable Protein Regulated by Insulin
      DescriptionA 117-amino acid protein substrate that is highly specific for MAP kinase. Phosphorylation of PHAS-I increases in response to insulin but not to cAMP stimulation of fat cells. An excellent substrate for MAP kinase both in vivo and in vitro. Reported to be a substrate for mammalian and yeast TOR phosphorylation.
      FormLiquid
      FormulationIn 50 mM NaCl, 25 mM Tris-HCl, 5 mM β-mercaptoethanol, 500 µM EDTA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Biological activityShown to undergo phosphorylation by activated MAP kinase (Cat. No. 454855) in the presence of ³²P-ATP.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesAlarcon, C.M., et al. 1999. Mol. Biol. Cell 10,2531. Hu, C., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 3730. Pause, A., et al. 1994. Nature 371, 762. Brunn, G.J., et al. 1991. Science 277, 99.