96-Well Plasmid and BAC Preparation Kit

Simply clear, concentrate, wash, and recover

Overview

Specifications

Ordering Information

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96-Well Plasmid and BAC Preparation Kit Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon Pack Sizeicon
LSKP09604Plasmid Miniprep96 Kit 4 Show Pricing & Availability
LSKP09624Plasmid Miniprep96 Kit 24 Show Pricing & Availability

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Accessories Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon Pack Sizeicon
LSKAST100Adhesive Tape Covers 100 Show Pricing & Availability
SCGVU01REStericup-GV, 0.22 µm, PVDF 12 Show Pricing & Availability

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Required Equiment Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon Pack Sizeicon
LSKVBP100V-bottom Plates 100 Show Pricing & Availability
MSVMHTS00MultiScreenHTS Vacuum Manifold 1 Show Pricing & Availability
WP6110060Chemical Duty Pump, 100 V/50–60 Hz 1 Show Pricing & Availability
WP6111560Chemical Duty Pump, 115 V/60 Hz 1 Show Pricing & Availability
WP6122050Chemical Duty Pump, 220 V/50 Hz 1 Show Pricing & Availability

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Reagents* Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon Volumeicon
LSKCLS500Cell Lysis Solution 0.5 L 500 mL Show Pricing & Availability
LSKCRS500Cell Resuspension Solution 0.5 L 500 mL Show Pricing & Availability
LSKCTB500Tris Buffer, 0.5 L 500 mL Show Pricing & Availability
LSKNF0500Nuclease-free Water, 0.5 L 500 mL Show Pricing & Availability
LSKNS0500Neutralization Solution, 0.5 L 500 mL Show Pricing & Availability
LSKPMRN30RNase A, 0.9 mL 0.9 mL Show Pricing & Availability

*All reagents are supplied 1 bottle/pack

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    Documentation

    Technical Info

    Title
    Fully Automated Method for Plasmid Mini-Preps Using Montage Plasmid Miniprep96 Kits on the Biomek® 2000
    Plasmid Minipreparation using MultiScreen 96-Well Filter Plates
    Tips to Enhance Operation of the Montage BAC96 Kit

    Data Sheet

    Title
    DNA Template Prep and Sequencing Reaction Cleanup
    Montage Plasmid Miniprep96 Kit

    FAQ

    QuestionAnswer
    Can I use this kit in centrifugal mode?Use of the Montage Plasmid Miniprep-96 Kit in a centrifugal protocol is not recommended.
    I'm using your two plate plasmid prep protocol and the wells are overflowing when I transfer the cleared lysate to the FB plate. What am I doing wrong?Overflowing can result from any of the following:
    • Using too much alkaline lysis and/or binding solutions.
    • Not removing all of the cell culture medium after pelleting cells. Tap the deep well block harder after decanting .
    • Cell pellet may be too large, as the result of overgrowth of the culture. Use a less rich medial like 2xLB.
    I have observed RNA contamination of my plasmid preps. Why?Failure to add RNase A to solution 1 will often result in RNA contamination in your DNA miniprep.
    I have observed lower than expected yields for my plasmid minpreps when using the Montage kit. Why?There are a number of factors that can contribute to low yields with the Montage kits they include: low/high copy number plasmid; bacterial strain: culturing time: inadequate cell resuspension: and overdrying of plasmid plate.
    I have observed that the wells of the MultiScreen plate used in the Montage kit are not filtering uniformly. Why?The wells of the MultiScreen plates usually filter uniformly. However, a bubble in the lysate may cause an airlock, which halts filtration. Also, a highly viscous sample may increase filtration time considerably.
    What is holdup volume of a MultiScreen Plate used with the Montage Kits?The holdup volume for the MultiScreen plate is between 5-20 microliters.
    When using the Montage kits how can I tell when filtration in the MultiScreen plate is complete?Filtration is complete when the individual wells of the MultiScreen plate are empty and the membranes appear shiny.
    The two sections of the MultiScreen plates used in the Montage kits are separating under vacuum. Why?The most common reason for this problem is the lack of a MultiScreen underdrain support grid in the vacuum manifold. A MultiScreen support grid is required for proper functioning of the MultiScreen plate with any vacuum manifold.
    The total yields with the Montage Plasmid Miniprep-96 kit seems to be less then with competitive products. Why?The Montage kit uses less culture volume (1ml vs. 1.3 ml) than most competitive kits. However the relative yield is equivalent.
    What is the total processing time for plasmid preparation using the Montage kits?Processing time depends on viscosity of sample, number of manifolds, familiarity with protocol, temperature. (Also, need vacuum capable of 24 in Hg or 812.7 millibar, 609.6 torr.)

    User Guides

    Title
    Beckman Compatible Base Kit for MultiScreen™ Solvent Resistant Vacuum Manifold
    Montage Plasmid Miniprep<sub>96</sub> Kit Laminated Card
    Montage Plasmid MiniprepHTS Kit User Guide
    MultiScreen HTS PLASMID 96-Well Plates User Guide
    MultiScreen Plasmid 384 Miniprep Clearing Plate