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ABE386 Anti-Flightless-1 homolog Antibody

Detect Fli1 using this rabbit polyclonal antibody, Anti-Flightless-1 homolog Antibody validated for use in western blotting & IP.

Anti-Flightless-1 homolog Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: FLII, FLIL UniProt Number: Q13045
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ABE386
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IPRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE386
      ReplacesMABF195; MABE530
      DescriptionAnti-Flightless-1 homolog Antibody
      Alternate Names
      • Protein flightless-1 homolog
      • Fli1
      • Anti-Flightless-1 homolog Antibody
      Background InformationMammalian homolog of drosophila flightless-1 is encoded by the FLII gene in human (also known as FLI, Fli1, or FLIL). The human flightless-1 homolog (Flii) contains sixteen 23-amino acid leucine-rich repeats (LRR) in its N-terminal half and a gelsolin-like domain (GLD) in its C-terminal half, composed of six segmental repeats typical of many gelsolin family members. Flii is reported to interact with actin and "FLI LRR associated protein" (FLAP) via its GLD and LRR domain, respectively (PMID 9525888). Flii is also shown to interact with the glucose-responsive transcription factor carbohydrate responsive element binding protein (ChREBP) via both the LRR and GLD domains and functions as a negative regulator of ChREBP in cancer cells (PMID 24055811). Increased Flii level is observed in the blistered skin of patients with epidermolysis bullosa acquisita (EBA), and Flii overexpression in transgenic mice results in delayed epidermal barrier formation in embryos and decreased expression of tight junction (TJ) proteins, as well as impaired barrier function among adult transgenic mice (PMID 24375017).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal in buffer containing serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Fli1 using this rabbit polyclonal antibody, Anti-Flightless-1 homolog Antibody validated for use in western blotting & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot of this antibody detected Flightless-1 homolog in IP (Prof. D. Pallas, Emory University).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Flightless-1 homolog.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FLII
      • FLIL
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~144 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flightless-1 homolog in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE386 04053252933868