UFC910096 | Amicon Ultra-15 Centrifugal Filter Unit

UFC910096
96  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsNMWLVolumeMinimum Final Concentrate Volume
      PExt, Protein Purification100 KDa15 mL200 µL
      Description
      Catalogue NumberUFC910096
      Trade Name
      • Amicon Ultra
      DescriptionAmicon Ultra-15 Centrifugal Filter Unit
      Background InformationAMICON ULTRA-15 15ML - 100 KDa cutoff. Available in 3 pack sizes.
      UFC910008 - 8 pack
      UFC910024 - 24 pack
      UFC910096 - 96 pack



      High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
      High retentate recovery of greater than 90%
      Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
      Heat-sealed membrane minimizes downstream extractables
      100% integrity tested for reliable performance
      Convenient sample monitoring with translucent housing and volume gradations
      Direct pipettor sample access eliminates processing step to recover concentrate
      High concentration factors of 80–100X

      Applications:
      Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
      Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
      Concentration of dilute or pre-purified proteins from column eluents
      Desalting, buffer exchange and protein dialysis
      References
      Product Information
      Filter CodePLHK
      Color CodeClear with pink cap
      Filter TypeUltrafiltration
      NMWL100 KDa
      Applications
      ApplicationUltracel-100 regenerated cellulose membrane, 15 mL sample volume
      Key Applications
      • Protein Extraction
      • Protein Purification
      Biological Information
      SterilityNon-Sterile
      Physicochemical Information
      Centrifugal ApplicationProtein Concentration and Desalting
      Dimensions
      Length12.1 cm (4.807 in.)
      Diameter2.97 cm
      Filtration Area7.6 cm²
      Minimum Final Concentrate Volume200 µL
      Volume15 mL
      Materials Information
      Chemistry
      • Regenerated Cellulose (RC)
      Device Material
      • Housing: Styrene/butadiene
      • Tube: Polypropylene
      • Cap and liner: Polyethylene
      Support MaterialRegenerated cellulose
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Packaging Information
      Material Size96
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Brochure

      Title
      Dialysis or diafiltration?
      Fast and Easy Sample Dialysis
      Protein purification and preparation

      FAQ

      QuestionAnswer
      How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
      Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
      What pipette or pipette tips do you recommend to recover the retentate from the Amicon Ultra concentrators?We do not have any recommendation for pipette or pipette tip in the insert-booklet of Amicon Ultra. Certain pipettes, like Eppendorf 100-1000µl, are very wide and the tip can not reach the bottom of the device. So a smaller pipette is needed. The pipette we use for all the studies is a Gilson pipetman with regular pipette tips 1-200µl from Fisher cat# 21-197-2J.
      Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
      What is the maximum sample volume for Amicon Ultra?
      Amicon Ultra-4Amicon Ultra-15
      Swinging Bucket4ml 15ml
      Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
      3.5ml(23 degree rotor angle)
      Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.
      Do you have any centrifugal filter units that are pyrogen free?We do not have any centrifugals devices that we claim are pyrogen free. Even the sterile devices could contain pyrogens.
      Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
      What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
      Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.

      User Guides

      Title
      Amicon® Ultra-15 Centrifugal Filter Devices User Guide