UFC901024 | Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane

UFC901024
24  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsNMWLVolumeMinimum Final Concentrate Volume
      PExt, Protein Purification10 KDa15 mL200 µL
      Description
      Catalogue NumberUFC901024
      Trade Name
      • Amicon Ultra
      DescriptionAmicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane
      References
      Product Information
      Filter CodePLGC
      Color CodeClear with pink cap
      Filter TypeUltrafiltration
      NMWL10 KDa
      Applications
      ApplicationThis Amicon Ultra 15 mL centrifugal filter enables the concentration of proteins with a membrane NMWL of 10 kDa. Amicon Ultra centrifugal filter units are ideal for protein purification, protein concentration & desalting.
      Key Applications
      • Protein Extraction
      • Protein Purification
      Biological Information
      SterilityNon-Sterile
      Physicochemical Information
      Centrifugal ApplicationProtein Concentration and Desalting
      Dimensions
      Length12.1 cm (4.807 in.)
      Diameter2.97 cm
      Filtration Area7.6 cm²
      Minimum Final Concentrate Volume200 µL
      Volume15 mL
      Materials Information
      Chemistry
      • Regenerated Cellulose (RC)
      Device Material
      • Housing: Styrene/butadiene
      • Tube: Polypropylene
      • Cap and liner: Polyethylene
      Support MaterialRegenerated cellulose
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Packaging Information
      Material Size24
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overviewPub Med ID
      Large-Scale Filter-Aided Sample Preparation Method for the Analysis of the Ubiquitinome.
      Casanovas A, Pinto-Llorente R, Carrascal M, Abian J
      Anal Chem  89(7)  3840-3846  2017

      Show Abstract
      28260372 28260372
      A complex of Protocadherin-19 and N-cadherin mediates a novel mechanism of cell adhesion.
      Emond, Michelle R, et al.
      J. Cell Biol., 195: 1115-21 (2011)  2011

      Show Abstract
      22184198 22184198

      Brochure

      Title
      Dialysis or diafiltration?
      Fast and Easy Sample Dialysis
      Protein purification and preparation
      Western Blotting Tools

      Posters

      Title
      A centrifugal ultrafiltration-based method for enrichment of microvesicles
      Preparing functional extracellular vesicles (EVs)

      FAQ

      QuestionAnswer
      Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
      What is the maximum sample volume for Amicon Ultra?
      Amicon Ultra-4Amicon Ultra-15
      Swinging Bucket4ml 15ml
      Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
      3.5ml(23 degree rotor angle)
      Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.
      Do you have any centrifugal filter units that are pyrogen free?We do not have any centrifugals devices that we claim are pyrogen free. Even the sterile devices could contain pyrogens.
      Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
      What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
      Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
      What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15?
      4ml15ml
      Swinging Bucket4000 x g4000 x g
      3500 x g for concentrated/viscous solutions
      Fixed Angle(23, 35 &45 degrees)7500 x g5000 x g
      What is the chemical compatibility of Amicon Ultra?Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.
      How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.

      User Guides

      Title
      Amicon® Ultra-15 10K Centrifugal Filter Devices
      Amicon® Ultra-15 10K centrifugos filtro prietaisas - Lietuviškai
      Amicon® Ultra-15 10K centrifugālās filtra ierīces - Latviski

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      Categories

      Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Sample Prep Centrifugal Filter Units