Amicon® Ultra-4 Centrifugal Filter Units

Provide both ultra-fast spin times and ultra-high recoveries

Overview

Specifications

Ordering Information

Amicon® Ultra-4 Centrifugal Filter Units Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon NMWLicon Pack Sizeicon
UFC800308Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-3 membrane 3 KDa 8 Show Pricing & Availability
UFC800324Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-3 membrane 3 KDa 24 Show Pricing & Availability
UFC800396Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-3 membrane 3 KDa 96 Show Pricing & Availability
UFC801008Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane 10 KDa 8 Show Pricing & Availability
UFC801024Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane 10 KDa 24 Show Pricing & Availability
UFC801096Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane 10 KDa 96 Show Pricing & Availability
UFC803008Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane 30 KDa 8 Show Pricing & Availability
UFC803024Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane 30 KDa 24 Show Pricing & Availability
UFC803096Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane 30 KDa 96 Show Pricing & Availability
UFC805008Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-50 membrane 50 KDa 8 Show Pricing & Availability
UFC805024Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-50 membrane 50 KDa 24 Show Pricing & Availability
UFC805096Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-50 membrane 50 KDa 96 Show Pricing & Availability
UFC810008Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-100 membrane 100 KDa 8 Show Pricing & Availability
UFC810024Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-100 membrane 100 KDa 24 Show Pricing & Availability
UFC810096Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-100 membrane 100 KDa 96 Show Pricing & Availability

Amicon Ultra-4 Filter Units are assembled with centrifuge tubes and caps.
The Amicon Ultra-4 with Ultracel-10 membrane is CE marked.

Back To Top

    Documentation

    Brochure

    Title
    Protein purification and preparation

    Posters

    Title
    Enhanced MALDI-TOF MS Spectra of Serum Peptides Using Ultrafiltration ; ASMS 2002

    References

    Reference overviewApplication
    Modification of Allergenicity and immunogenicity of formate dehydrogenase by conjugation with linear mono methoxy poly ethylene glycol: Improvement in detoxification of formate in methanol poisoning
    Muthuvel A., Rajamani R., Senthilvelan M., Manikandan s., Sheeladevi R.
    Clinica Chimica acta 374 (2006) 122-128  2006

    Protein Purification
    Simultaneous reduction and alkylation of protein disulfides in a centrifugal ultrafiltration device prior to two-dimensional gel electrophoresis.
    Smejkal GB, Li C, Robinson MH, Lazarev AV, Lawrence NP, Chernokalskaya E.
    J Proteome Res. 2006 Apr;5(4):983-7.  2006

    Protein Concentration
    Measurement of Pro-C-Type Natriuretic Peptide in Plasma
    Nielsen, S.J., Rehfeld, J.F., Pedersen, F., Kastrup, J., Videback, R., Goetze, J.P.
    Clinical Chemistry 51 (2005): 2173-2716  2005

    Comparative Proteomic Analysis of Intra- and Interindividual Variation in Human Cerebrospinal Fluid
    Hu, Y., Malone, J.P., Fagan, A.M., Townsend, R.R., Holtzmann, D.M
    Molecular & Cellular Proteomics 4.12 (2005): 2000-2009  2005

    Ultrafiltration for proteomic sample preparation
    E. Chernokalskaya, S. Gutierrez, A.M. Pit, J. T. Leonard
    Electrophoresis, 25: 2461-2468  2004

    Sample Prep

    FAQ

    QuestionAnswer
    Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
    What is the maximum sample volume for Amicon Ultra?
    Amicon Ultra-4Amicon Ultra-15
    Swinging Bucket4ml 15ml
    Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
    3.5ml(23 degree rotor angle)
    Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.
    Do you have any centrifugal filter units that are pyrogen free?We do not have any centrifugals devices that we claim are pyrogen free. Even the sterile devices could contain pyrogens.
    Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
    What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
    Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
    What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15?
    4ml15ml
    Swinging Bucket4000 x g4000 x g
    3500 x g for concentrated/viscous solutions
    Fixed Angle(23, 35 &45 degrees)7500 x g5000 x g
    What is the chemical compatibility of Amicon Ultra?Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.
    How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.

    User Guides

    Title
    Amicon® Ultra-4 Centrifugal Filter Devices User Guide
    Amicon® Ultra-4 Centrifugal Filter Devices User Guide
    Amicon® Ultra-4 centrifugālās filtra ierīces- Latviski
    Amicon® Ultra-4" centrifugos filtro prietaisas - Lietuviškai