05-1425 | Anti-14-3-3 Antibody, 33 kDa isoforms, clone 8C3

500 µL  
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      Replacement Information
      Replacement Information04-1089 is the recommended replacement for Discontinued catalog number 05-1425

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, Sh, HWBMAscitesMonoclonal Antibody
      Catalogue Number05-1425
      DescriptionAnti-14-3-3 Antibody, 33 kDa isoforms, clone 8C3
      Alternate Names
      • 14-3-3 epsilon
      • 14-3-3 protein epsilon, mitochondrial import stimulation factor L subunit
      • protein kinase C inhibitor protein-1
      • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
      • tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide 2
      Background InformationMammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's.
      Product Information
      • Jurkat cell lysate
      PresentationAscites. Liquid containing 0.1% sodium azide.
      ApplicationThis Anti-14-3-3 Antibody, 33 kDa isoforms, clone 8C3 is validated for use in WB for the detection of 14-3-3.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenDirected against a synthetic peptide corresponding to rat 14-3-3 epsilon, amino acids 208-244.
      Epitopea.a. 208-244
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityClone 8C3 is specific for the 33 kDa epsilon isoform of 14-3-3 proteins and does not cross react with the 30 kDa 14-3-3 isoforms
      Species Reactivity
      • Rat
      • Sheep
      • Human
      Species Reactivity NoteReacts with human. Expected to react in rat and sheep.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • YWHAE
      • KCIP-1
      • MDS
      • 14-3-3E
      • MDCR
      • FLJ45465
      Purification MethodUnpurified, ascites liquid.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62258 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
      SIZE: 255 amino acids; 29174 Da
      SUBUNIT: Homodimer. Interacts with NDEL1 (By similarity). Interacts with HCV core protein.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      SIMILARITY: SwissProt: P62258 ## Belongs to the 14-3-3 family.
      Molecular Weight33 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysates.
      Western Blot Analysis: 1:500 dilution of this lot detected 14-3-3 PROTEINS on 10 ug of Jurkat lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies