04-321 | Anti-4E-BP1 Antibody, rabbit monoclonal

04-321
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, WB, IH(P)RbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-321
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-4E-BP1 Antibody, rabbit monoclonal
      Alternate Names
      • EIF4EBP1
      • eIF4E-binding protein 1
      • eukaryotic translation initiation factor 4E binding protein 1
      References
      Product Information
      FormatPurified
      Presentation100 μL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 0.09 M NaCl, 0.006% sodium azide and 0.03% BSA and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-4E-BP1 Antibody, Rabbit is an antibody against 4E-BP1 for use in FC, WB, IH(P).
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the C-terminal region of human 4E-BP1 (eIF4E-binding protein).
      HostRabbit
      SpecificityRecognizes the C-terminal domain of 4E-BP1.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteNot expected to react with mouse or rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
      Gene Symbol
      • EIF4EBP1
      • PHAS-I
      • 4EBP1
      • MGC4316
      • 4E-BP1
      • BP-1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13541 # Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.
      SIZE: 118 amino acids; 12580 Da
      SUBUNIT: Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.
      PTM: Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: Q13541 ## Belongs to the eIF4E-binding protein family.
      Molecular Weight15-20 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-4E-BP1, Rabbit Monoclonal (Rabbit monoclonal IgG) - 2066300 2066300
      Anti-4E-BP1, Rabbit Monoclonal - 0611045805 0611045805
      Anti-4E-BP1, Rabbit Monoclonal - 1979243 1979243
      Anti-4E-BP1, Rabbit Monoclonal - 2069342 2069342
      Anti-4E-BP1, Rabbit Monoclonal - 2542043 2542043
      Anti-4E-BP1, Rabbit Monoclonal - DAM1535705 DAM1535705

      References

      Reference overviewPub Med ID
      eIF4E binding protein 1 and H-Ras are novel substrates for the protein kinase activity of class-I phosphoinositide 3-kinase
      Foukas, Lazaros C and Shepherd, Peter R
      Biochem Biophys Res Commun, 319:541-9 (2004)  2004

      15178440 15178440
      The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif
      Nojima, Hiroki, et al
      J Biol Chem, 278:15461-4 (2003)  2003

      12604610 12604610
      Insulin activation of Rheb, a mediator of mTOR/S6K/4E-BP signaling, is inhibited by TSC1 and 2
      Garami, Attila, et al
      Mol Cell, 11:1457-66 (2003)  2003

      12820960 12820960

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies