05-725 | Anti-53BP1 Antibody, clone BP18

05-725
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBMAscitesMonoclonal Antibody
      Description
      Catalogue Number05-725
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-53BP1 Antibody, clone BP18
      References
      Product Information
      FormatAscites
      Presentationmouse ascites IgM containing 0.05% sodium azide and 30% glycerol
      Applications
      ApplicationDetect 53BP1 with Anti-53BP1 Antibody, clone BP18 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenMix of three GST fusion proteins corresponding to residues 1-337, 338-671, and 1331-1664, respectively, of human 53BP1
      Cloneclone BP18
      HostMouse
      Specificity53BP1
      IsotypeIgM
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TP53BP1
      • 53BP1
      • p53BP1
      • p202
      • FLJ41424
      • MGC138366
      Purification MethodAscites
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q12888 # May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Binds to sites of DNA damage. Plays a role in the response to DNA damage.
      SIZE: 1972 amino acids; 213574 Da
      SUBUNIT: Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H3 that has been methylated on 'Lys-79'. Does not bind unmethylated histone H3.
      SUBCELLULAR LOCATION: Nucleus. Kinetochore. Note=Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks.
      PTM: Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. & Phosphorylated in an ATM-dependent manner in response to DNA damage.
      SIMILARITY: SwissProt: Q12888 ## Contains 2 BRCT domains.
      Molecular Weight~250kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on whole cell lysates from HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-53BP1, clone BP18 2472932
      Anti-53BP1, clone BP18 (mouse ascites IgM) 2879674
      Anti-53BP1, clone BP18 - 24567 24567

      References

      Reference overviewPub Med ID
      p53 Binding protein 53BP1 is required for DNA damage responses and tumor suppression in mice.
      Ward, Irene M, et al.
      Mol. Cell. Biol., 23: 2556-63 (2003)  2003

      Show Abstract
      12640136 12640136
      Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways.
      Rappold, I, et al.
      J. Cell Biol., 153: 613-20 (2001)  2001

      Show Abstract
      11331310 11331310

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies