04-150 | Anti-AIRE Antibody, clone 5H12

04-150
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, WB, ICC, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-150
      DescriptionAnti-AIRE Antibody, clone 5H12
      Alternate Names
      • Autoimmune regulator
      • Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog
      • APECED protein homolog
      • AIRE
      Background InformationAIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2cκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect AIRE using this rat monoclonal antibody, Anti-AIRE Antibody, clone 5H12 validated for use in Flow Cytometry, western blotting, ICC & IHC.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A representative lot from an independent laboratory detected AIRE in AIRE transfected COS cells (Hubert, F. X., et al. (2008). J Immunol. 180(6):3824-3832.).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected AIRE in mouse thymus sections (Hubert, F. X., et al. (2008). J Immunol. 180(6):3824-3832.).

      Immunocytochemistry Analysis: A representative lot from an independent laboratory detected AIRE in mouse thymic epithelial cells (Guerau-de-Arellano, M., et al. (2009). J Exp Med. 206(6):1245-1252.).
      Biological Information
      ImmunogenLinear peptide corresponding to the C-terminus of mouse AIRE.
      Clone5H12
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG2cκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Aire
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight60 kDa calculated. Many isoforms are known to exist.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in mouse primary splenocytes.

      Flow Cytometry Analysis: 4 µg of this antibody detected AIRE in 1X10E6 mouse primary splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-AIRE, clone 5H12 - Q2315811 Q2315811
      Anti-AIRE, clone 5H12 -2603258 2603258
      Anti-AIRE, clone 5H12 -2855634 2855634

      References

      Reference overviewPub Med ID
      Neonatal tolerance revisited: a perinatal window for Aire control of autoimmunity.
      Guerau-de-Arellano, Mireia, et al.
      J. Exp. Med., 206: 1245-52 (2009)  2009

      Show Abstract
      19487417 19487417
      A specific anti-Aire antibody reveals aire expression is restricted to medullary thymic epithelial cells and not expressed in periphery.
      Hubert, François-Xavier, et al.
      J. Immunol., 180: 3824-32 (2008)  2008

      Show Abstract
      18322189 18322189

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