09-882 Anti-ALKBH3 Antibody

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number09-882
      DescriptionAnti-ALKBH3 Antibody
      Alternate Names
      • Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
      • Alkylated DNA repair protein alkB homolog 3
      • DEPC-1
      • Prostate cancer antigen 1
      Background InformationABH3 is a member of the Fe(II)/2OG-dependent dioxygenase superfamily, which couples substrate oxidation by converting 2-oxoglutarate (2OG) into succinate and CO2. In humans, ABH3 plays a critical role in alkylated DNA/RNA damage repair and in cancer therapy. While ABH3 may repair alkylated RNA, it demonstrates greater preference for the repair of single- over double-stranded alkylated DNA through oxidative demethylation of 1-methyladenine and 3-methylcytosine.
      Product Information
      FormatAffinity Purified
      • Human prostate tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationUse Anti-ALKBH3 Antibody (rabbit polyclonal antibody) validated in WB to detect ALKBH3 also known as Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: A representative lot from an independent laboratory detected ALKBH3 in PC-3 and LNCap whole cell extract. (Dango, S., et al. (2011). Mol Cell. {Epub ahead of print.})
      Biological Information
      ImmunogenHistidine-tagged-recombinant protein corresponding to the the N-terminus of human ALKBH3.
      SpecificityThis antibody recognizes the N-terminus of ALKBH3.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Other homologies Orangutan 98% and Rhesus Monkey 97% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine.
      Gene Symbol
      • ALKBH3
      • ABH3
      • DEPC1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Dioxygenase that repairs alkylated DNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has a strong preference for single-stranded DNA. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.

      COFACTOR: Binds 1 Fe2+ ion per subunit.

      ENZYME REGULATION: Activated by ascorbate.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.

      TISSUE SPECIFICITY: Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.
      Molecular Weight~35 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human prostate tissue lysate.

      Western Blot Analysis: A 1:5,000 dilution of this antibody detected ALKBH3 in human prostate tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ALKBH3 - 22954952295495
      Anti-ALKBH3 - 23615512361551
      Anti-ALKBH3 - NRG1948876NRG1948876
      Anti-ALKBH3 -25712812571281
      Anti-ALKBH3 -26017372601737
      Anti-ALKBH3 -27726532772653
      Anti-ALKBH3 -28092862809286
      Anti-ALKBH3 Polyclonal Antibody2910161
      Anti-ALKBH3 Polyclonal Antibody2955693
      Anti-ALKBH3 Polyclonal Antibody3012261


      Reference overviewPub Med ID
      The oxidative demethylase ALKBH3 marks hyperactive gene promoters in human cancer cells.
      Liefke, R; Windhof-Jaidhauser, IM; Gaedcke, J; Salinas-Riester, G; Wu, F; Ghadimi, M; Dango, S
      Genome medicine  7  66  2015

      Show Abstract
      26221185 26221185
      DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation.
      Dango, S; Mosammaparast, N; Sowa, ME; Xiong, LJ; Wu, F; Park, K; Rubin, M; Gygi, S; Harper, JW; Shi, Y
      Molecular cell  44  373-84  2011

      Show Abstract
      22055184 22055184


      New Products: Volume 3, 2012