07-363 Anti-AMPK α2 Antibody

07-363
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-363
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-AMPK α2 Antibody
      Alternate Names
      • AMP-Activated Protein Kinase
      • AMPK
      References
      Product Information
      FormatPurified
      Control
      • Rat liver preparation
      PresentationProtein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationDetect AMPK α2 using this Anti-AMPK α2 Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to amino acids 353-367 (C-DDSAMHIPPGLKPHP) of human AMP-Activated Protein Kinase alpha2 (AMPKalpha2) catalytic subunit
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes AMP-Activated Protein Kinase alpha2 (AMPKα2).
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
      Gene Symbol
      • PRKAA2
      • AMPK
      • AMPK2
      • PRKAA
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P54646 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
      COFACTOR: Magnesium.
      SIZE: 552 amino acids; 62320 Da
      SUBUNIT: Heterotrimer of a catalytic subunit, a beta and a gamma non-catalytic subunits.
      SIMILARITY: SwissProt: P54646 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
      Molecular Weight~63 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot in a rat liver preparation
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-AMPK a2 (rabbit polyclonal IgG)2900286
      Anti-AMPK a2 (rabbit polyclonal IgG)2950557
      Anti-AMPK alpha;2 - 2242922429
      Anti-AMPK alpha;2 - 3208232082
      Anti-AMPK α2 (rabbit polyclonal IgG)3124164
      Anti-AMPK α2 (rabbit polyclonal IgG)2475668
      Anti-AMPKα2 Polyclonal Antibody2872326
      Anti-AMPKα2 Polyclonal Antibody2830166
      Anti-AMPKα2 Polyclonal Antibody2935473

      References

      Reference overviewApplicationPub Med ID
      Data in support of 5'AMP-activated protein kinase alpha regulates stress granule biogenesis.
      Mahboubi, H; Barisé, R; Stochaj, U
      Data in brief  4  54-9  2015

      Show Abstract
      26217763 26217763
      AMPK-dependent phosphorylation of ULK1 regulates ATG9 localization.
      Mack, HI; Zheng, B; Asara, JM; Thomas, SM
      Autophagy  8  1197-214  2012

      Show Abstract
      22932492 22932492
      Comparing the effects of nano-sized sugarcane fiber with cellulose and psyllium on hepatic cellular signaling in mice.
      Wang, Zhong Q, et al.
      Int J Nanomedicine, 7: 2999-3012 (2012)  2012

      Show Abstract
      22787396 22787396
      Myo1c regulates glucose uptake in mouse skeletal muscle.
      Toyoda, T; An, D; Witczak, CA; Koh, HJ; Hirshman, MF; Fujii, N; Goodyear, LJ
      The Journal of biological chemistry  286  4133-40  2011

      Show Abstract
      21127070 21127070
      Transcriptome and translational signaling following endurance exercise in trained skeletal muscle: impact of dietary protein.
      Rowlands, DS; Thomson, JS; Timmons, BW; Raymond, F; Fuerholz, A; Mansourian, R; Zwahlen, MC; Métairon, S; Glover, E; Stellingwerff, T; Kussmann, M; Tarnopolsky, MA
      Physiological genomics  43  1004-20  2011

      Show Abstract
      21730029 21730029
      TBC1D1 regulates insulin- and contraction-induced glucose transport in mouse skeletal muscle.
      An, D; Toyoda, T; Taylor, EB; Yu, H; Fujii, N; Hirshman, MF; Goodyear, LJ
      Diabetes  59  1358-65  2010

      Show Abstract Full Text Article
      Western Blotting, Immunoprecipitation20299473 20299473
      Alpha2-AMPK activity is not essential for an increase in fatty acid oxidation during low-intensity exercise.
      Miura, S; Kai, Y; Kamei, Y; Bruce, CR; Kubota, N; Febbraio, MA; Kadowaki, T; Ezaki, O
      American journal of physiology. Endocrinology and metabolism  296  E47-55  2009

      Show Abstract
      18940938 18940938
      TBC 11251. IPI 1040.
      [No authors listed]
      Drugs in RD  2  38-9  2001

      10610280 10610280
      Dealing with energy demand: the AMP-activated protein kinase.
      Kemp, B E, et al.
      Trends Biochem. Sci., 24: 22-5 (1999)  1999

      Show Abstract
      10087918 10087918
      Isoform-specific purification and substrate specificity of the 5'-AMP-activated protein kinase.
      Michell, B J, et al.
      J. Biol. Chem., 271: 28445-50 (1996)  1996

      Show Abstract
      8910470 8910470