04-323 | Anti-AMPKα1 Antibody, rabbit monoclonal

04-323
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIH(P), IP, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-323
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-AMPKα1 Antibody, rabbit monoclonal
      Alternate Names
      • PRKAA1
      • protein kinase, AMP-activated, alpha 1 catalytic subunit
      • AMPK
      • AMPKa1
      • AMPK alpha 1
      References
      Product Information
      FormatPurified
      PresentationIn 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect AMPKα1 using this Anti-AMPKα1 Antibody, Rabbit validated for use in IH(P), IP & WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the C-terminal region of human AMPKα1.
      HostRabbit
      SpecificityRecognizes the C-terminal domain of AMPKα1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
      Gene Symbol
      • PRKAA1
      • MGC57364
      • AMPK
      • AMPK1
      • MGC33776
      • AMPKa1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13131 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
      COFACTOR: Magnesium.
      SIZE: 550 amino acids; 62808 Da
      SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1.
      SIMILARITY: SwissProt: Q13131 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
      Molecular Weight63 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-AMPK#945;1, Rabbit Monoclonal (Rabbit monoclonal IgG) - 2111381 2111381
      Anti-AMPK#945;1, Rabbit Monoclonal (Rabbit monoclonal IgG) - 2115543 2115543
      Anti-AMPK#945;1, Rabbit Monoclonal (Rabbit monoclonal IgG) - 2118896 2118896
      Anti-AMPK#945;1, Rabbit Monoclonal (Rabbit monoclonal IgG) - 2151825 2151825
      Anti-AMPKa1, Rabbit Monoclonal (Rabbit monoclonal IgG) - 2279490 2279490
      Anti-AMPKa1, Rabbit Monoclonal - NG1870407 NG1870407
      Anti-AMPKalpha;1, Rabbit Monoclonal - 0612047410 0612047410
      Anti-AMPKalpha;1, Rabbit Monoclonal - DAM1418685 DAM1418685
      Anti-AMPKα1, Rabbit Monoclonal 2475591
      Anti-AMPKα1, Rabbit Monoclonal (Rabbit monoclonal IgG) - 2424647 2424647

      References

      Reference overviewPub Med ID
      AMPK-dependent phosphorylation of ULK1 regulates ATG9 localization.
      Mack, HI; Zheng, B; Asara, JM; Thomas, SM
      Autophagy  8  1197-214  2012

      Show Abstract
      22932492 22932492
      TSC2 integrates Wnt and energy signals via a coordinated phosphorylation by AMPK and GSK3 to regulate cell growth.
      Inoki, Ken, et al.
      Cell, 126: 955-68 (2006)  2006

      Show Abstract
      16959574 16959574
      Glucose metabolism and cancer
      Shaw, Reuben J
      Curr Opin Cell Biol, 18:598-608 (2006)  2006

      17046224 17046224
      AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through down-regulated mammalian target of rapamycin (mTOR) signaling
      Bolster, Douglas R, et al
      J Biol Chem, 277:23977-80 (2002)  2002

      11997383 11997383

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies