04-324 | Anti-AMPKβ1 Antibody, rabbit monoclonal

04-324
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, IH(P), IP, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-324
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-AMPKβ1 Antibody, rabbit monoclonal
      Alternate Names
      • PRKAB1
      • AMPK
      • AMPKb1
      • AMPK beta 1
      • protein kinase, AMP-activated, beta 1 non-catalytic subunit
      References
      Product Information
      FormatPurified
      PresentationIn 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationAnti-AMPKβ1 Antibody, Rabbit detects level of AMPKβ1 & has been published & validated for use in FC, IH(P), IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to human AMPKβ1.
      HostRabbit
      SpecificityRecognizes AMPKβ1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
      Gene Symbol
      • PRKAB1
      • AMPKb
      • AMPK
      • HAMPKb
      • MGC17785
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9Y478 # AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. Also regulates cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase and hormone- sensitive lipase. This is a regulatory subunit, may be a positive regulator of AMPK activity. It may also serve as an adaptor molecule for the catalytic alpha-subunit.
      SIZE: 270 amino acids; 30382 Da
      SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic regulatory subunits. Interacts with FNIP1.
      PTM: Phosphorylated (By similarity).
      SIMILARITY: SwissProt: Q9Y478 ## Belongs to the 5'-AMP-activated protein kinase beta subunit family.
      Molecular Weight38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-AMPKb1, Rabbit Monoclonal (Rabbit monoclonal IgG) - 2246757 2246757
      Anti-AMPKb1, Rabbit Monoclonal (Rabbit monoclonal IgG) - 2266442 2266442
      Anti-AMPKb1, Rabbit Monoclonal (Rabbit monoclonal IgG) - 2299125 2299125
      Anti-AMPKb1, Rabbit Monoclonal - 2038233 2038233
      Anti-AMPKbeta;1, Rabbit Monoclonal - 0611045563 0611045563
      Anti-AMPKβ1, Rabbit Monoclonal - 2435040 2435040

      References

      Reference overviewPub Med ID
      TSC2 integrates Wnt and energy signals via a coordinated phosphorylation by AMPK and GSK3 to regulate cell growth.
      Inoki, Ken, et al.
      Cell, 126: 955-68 (2006)  2006

      Show Abstract
      16959574 16959574
      Glucose metabolism and cancer
      Shaw, Reuben J
      Curr Opin Cell Biol, 18:598-608 (2006)  2006

      17046224 17046224
      AMP-activated protein kinase suppresses protein synthesis in rat skeletal muscle through down-regulated mammalian target of rapamycin (mTOR) signaling
      Bolster, Douglas R, et al
      J Biol Chem, 277:23977-80 (2002)  2002

      11997383 11997383