05-704 | Anti-ARNT1/HIF-1β Antibody, clone 4G9

05-704
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-704
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-ARNT1/HIF-1β Antibody, clone 4G9
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect ARNT1/HIF-1 beta with Anti-ARNT1/HIF-1β Antibody, clone 4G9 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenProtein A fusion protein corresponding to residues 399-777 of human aryl hydrocarbon receptor nuclear translocator (ARNT)
      Cloneclone 4G9
      HostMouse
      SpecificityARNT
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene.
      Gene Symbol
      • ARNT
      • HIF-1beta
      • HIF1beta
      • HIF1B
      • HIF1BETA
      • TANGO
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P27540 # Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
      SIZE: 789 amino acids; 86636 Da
      SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with AHR, with HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. Interacts with TACC3 (By similarity). Interacts with NOCA7.
      SUBCELLULAR LOCATION: Nucleus.
      SIMILARITY: SwissProt: P27540 ## Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 2 PAS (PER-ARNT-SIM) domains.
      Molecular Weight87kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on lysates from HeLa, A431, 3T3/A31, or Jurkat cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ARNT, clone 4G9 -2517340 2517340
      Anti-ARNT, clone 4G9 -2743610 2743610
      Anti-ARNT1/HIF-1beta;, clone 4G9 - 24418 24418

      References

      Reference overviewPub Med ID
      Chromium cross-links histone deacetylase 1-DNA methyltransferase 1 complexes to chromatin, inhibiting histone-remodeling marks critical for transcriptional activation.
      Schnekenburger, M; Talaska, G; Puga, A
      Molecular and cellular biology  27  7089-101  2007

      Show Abstract Full Text Article
      17682057 17682057
      Physicochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocator: characterization of two monoclonal antibodies to the aryl hydrocarbon receptor nuclear translocator.
      Hord, N G and Perdew, G H
      Mol. Pharmacol., 46: 618-26 (1994)  1994

      Show Abstract
      7526149 7526149

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies