06-466 | Anti-Abl SH3 Domain Antibody

06-466
250 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-466
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Abl SH3 Domain Antibody
      OverviewThe amount of c-Abl tends to be low in many cell lines and c-Abl was not detected using RIPA lysates from human Jurkat, Raj, Hut lymphoid cell lines, or murine Wehi myeloid cell line
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine buffer, pH 7.4
      Applications
      ApplicationAnti-Abl SH3 Domain Antibody detects level of Abl SH3 Domain & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenRecombinant protein containing GST fused to the SH3 domain of human p210bcr-abl corresponding to amino acids 929-1020
      HostRabbit
      Specificityhuman p210bcr-abl, p140c-Abl, as well as viral p160gag-abl
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11.
      Gene Symbol
      • ABL1
      • v-abl
      • JTK7
      • p150
      • c-ABL
      • bcr/abl
      • ABL
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00519 # Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.
      COFACTOR: Magnesium or manganese.
      SIZE: 1130 amino acids; 122873 Da
      SUBUNIT: Interacts with SORBS1 following insulin stimulation. Found in a trimolecular complex containing CDK5 and CABLES1. Interacts with CABLES1 and PSTPIP1. Interacts with ZDHHC16 (By similarity). Interacts with INPPL1/SHIP2.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=The myristoylated c-ABL protein is reported to be nuclear.
      TISSUE SPECIFICITY: Widely expressed.
      PTM: Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation. Isoform IB is myristoylated on Gly-2.
      DISEASE: SwissProt: P00519 # A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
      SIMILARITY: SwissProt: P00519 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight140kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on human K562 leukemia cells or NFC murine erythroleukemia cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti- Abl SH3 domain (rabbit polyclonal IgG) - 2322035 2322035
      Anti- Abl SH3 domain - DAM1725584 DAM1725584
      Anti- Abl SH3 domain - DAM1770292 DAM1770292
      Anti-Abl SH3 Domain - 0611045710 0611045710
      Anti-Abl SH3 Domain - 14336 14336
      Anti-Human Abl SH3 domain (rabbit polyclonal IgG) - DAM1460248 DAM1460248

      References

      Reference overviewPub Med ID
      Comparison of various kinds of bone marrow stem cells for the repair of infarcted myocardium: single clonally purified non-hematopoietic mesenchymal stem cells serve as a superior source.
      Shaoheng Zhang, Junbo Ge, Aijun Sun, Danling Xu, Juying Qian, Jinyi Lin, Yan Zhao, Haifeng Hu, Ying Li, Keqiang Wang, Yunzeng Zou
      Journal of cellular biochemistry  99  1132-47  2006

      Show Abstract
      16795039 16795039
      Bcr-Abl resistance screening predicts a limited spectrum of point mutations to be associated with clinical resistance to the Abl kinase inhibitor nilotinib (AMN107).
      von Bubnoff, N; Manley, PW; Mestan, J; Sanger, J; Peschel, C; Duyster, J
      Blood  108  1328-33  2006

      Show Abstract
      16614241 16614241
      Calpain regulation of cytoskeletal signaling complexes in von Willebrand factor-stimulated platelets. Distinct roles for glycoprotein Ib-V-IX and glycoprotein IIb-IIIa (integrin alphaIIbbeta3) in von Willebrand factor-induced signal transduction.
      Y Yuan,S M Dopheide,C Ivanidis,H H Salem,S P Jackson
      The Journal of biological chemistry  272  1997

      Show Abstract
      9268316 9268316
      Abl tyrosine protein kinase.
      Laneuville, P
      Semin. Immunol., 7: 255-66 (1995)  1995

      Show Abstract
      8520030 8520030
      Abl-mediated transformation, immunoglobulin gene rearrangements and arrest of B lymphocyte differentiation.
      Rosenberg, N
      Semin. Cancer Biol., 5: 95-102 (1994)  1994

      Show Abstract
      8061334 8061334
      T-cell immunity to oncogenic proteins including mutated ras and chimeric bcr-abl.
      Cheever, M A, et al.
      Ann. N. Y. Acad. Sci., 690: 101-12 (1993)  1993

      Show Abstract
      8103658 8103658
      Abl tyrosine kinase in signal transduction and cell-cycle regulation.
      Wang, J Y
      Curr. Opin. Genet. Dev., 3: 35-43 (1993)  1993

      Show Abstract
      8453272 8453272
      Induction of mouse thymocyte apoptosis by inhibitors of tyrosine kinases is associated with dephosphorylation of nuclear proteins.
      Y Azuma,Y Onishi,Y Sato,H Kizaki
      Cellular immunology  152  1993

      Show Abstract
      8242767 8242767

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies