CBL171-I | Anti-Actin, smooth muscle Antibody, clone ASM-1/1A4

CBL171-I
100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Ch, BWB, IHC, ICC, IF, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL171-I
      DescriptionAnti-Actin, smooth muscle Antibody, clone ASM-1/1A4
      Alternate Names
      • Actin, aortic smooth muscle
      • Alpha-actin-2
      • Cell growth-inhibiting gene 46 protein
      Background InformationActin, aortic smooth muscle (UniProt P62736; also known as Alpha-actin-2, Cell growth-inhibiting gene 46 protein) is encoded by the ACTA2 (also known as AAT6, ACTSA, ACTVS, GIG46, MYMY5) gene (Gene ID 59) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Actin, smooth muscle Antibody, clone ASM-1/1A4 is an antibody against Actin for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      • Immunofluorescence
      • ELISA
      Application NotesWestern Blotting Analysis: A representative lot detected full-length and caspse-3-cleaved smooth muscle actin in uterus extracts from pregnant mice (Jeyasuria, P., et al. (2009). Biol. Reprod. 80(5):928-934).
      Western Blotting Analysis: A representative lot detected smooth muscle actin in PC-3M-LN4 and PC-3M-LN4-derived human prostate cancer cells (Zvieriev, V., et al. (2005). Biochem. Biophys. Commun. 337(2):498-504).
      Western Blotting Analysis: A representative lot detected developing stage-dependent vascular alpha-actin level in chicken heart of various somite stages (Ehler, E., et al. (2004). Dev. Dyn. 229(4):745-755).
      Western Blotting Analysis: A representative lot detected aortic actin, but not actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), or myocardium (alpha-myocardial) in various human, rat, bovine and rabbit tissue and cell lysates (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
      Immunohistochemistry Analysis: A representative lot immunostained smooth muscle actin-positive myofibroblasts in paraformaldehyde-fix rat skin wounds sections (Mirastschijski, U., et al. (2010). Wound Repair Regen. 18(2):223-234).
      Immunohistochemistry Analysis: A representative lot immunostained activated myofibroblasts in formalin-fixed, paraffin-embedded mouse submandibular salivary gland (Hall, B.E., et al. (2010). Lab. Invest. 90(4):543-555).
      Immunohistochemistry Analysis: A representative lot immunostained α-SMA-positive cells in paraffin-embedded rat healing patellar tendon sections (Fu, S.C., et al. (2008). J. Orthop. Res. 26(3):374-383).
      Immunocytochemistry Analysis: Representative lots immunostained the mesoderm layer of embryoid bodies formed in vitro from northern white rhinoceros iPSCs, as well as murine iPSCs and mESCs (Ben-Nun, I.F., et al. (2011). Nat. Methods. 8(10):829-831; Gerlach, J.C., et al. (2010). Cells Tissues Organs. 192(1):39-49; Shao, L., et al. (2009). Cell Res. 19(3):296-306).
      Immunohistochemistry Analysis: A representative lot immunostained a subsets of cultured rat aortic medial smooth muscle cells (SMCs) and dermal fibroblasts (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
      Immunohistochemistry Analysis: A representative lot immunostained cultured rat tendon cells (Fu, S.C., et al. (2008). J. Orthop. Res. 26(3):374-383).
      Immunofluorescence Analysis: A representative lot immunostained myofibrils using methanol-fixed, 9-somite stage chicken heart whole-mount preparations. The vascular alpha-actin staining started to disappear from a subset of the myofibrils when using 10-somite stage chicken heart preparations (Ehler, E., et al. (2004). Dev. Dyn. 229(4):745-755).
      Immunofluorescence Analysis: A representative lot immunostained stromal vessel SM cells in acetone-fixed benign (leiomyomas) and malignant (leiomyosarcomas & intravascular leiomyomatosis) smooth muscle (SM) neoplasms cryosections (Schürch, W., et al. (1987). Am. J. Pathol. 128(1):91-103).
      Immunofluorescence Analysis: A representative lot immunostained chicken gizzard and rat myocardium blood vessels, as well as smooth muscle cells (SMCs) in various acetone-fixed human and rat cryosections, whereas chicken gizzard parenchyrnal cells, rat cardiocytes, aorta endothelial cells and adventitial fibroblasts were negative (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
      ELISA Analysis: A representative lot detected aortic actin, but not actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), or myocardium (alpha-myocardial) by ELISA (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
      Biological Information
      ImmunogenBSA-conjugated linear peptide corresponding to the N-terminal sequence of human smooth muscle actin.
      EpitopeN-terminus.
      CloneASM-1 (1A4)
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone ASM-1, also known as anti-αsm-1 and clone 1A4, reacted with aortic actin, but not actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial) by ELISA and Western blotting (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Chicken
      • Bovine
      Species Reactivity NoteHuman, Mouse, Rat, Chicken, Bovine. Predicted to react with Equine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ACTA2
      • AAT6
      • ACTSA
      • ACTVS
      • GIG46
      • MYMY5
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~45 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HUVEC lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected smooth muscle actin in 10 µg of HUVEC lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      ABT1487 Anti-alpha-Actin Antibody, Smooth Muscle Show Pricing & Availability
      CBL171 Anti-Actin Antibody, smooth muscle, clone ASM-1 Show Pricing & Availability
      MABT381 Anti-Alpha-Actin (smooth muscle) Antibody, clone EPR5368, rabbit monoclonal Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies