07-590 | Anti-Ago 2 Antibody

07-590
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-590
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Ago 2 Antibody
      Background InformationThis gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

      Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate, HL-60 cell lysate
      PresentationProtein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-Ago 2 Antibody is a Rabbit Polyclonal Antibody for detection of Ago 2 also known as argonaute 2 & has been tested in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 7-48 of human argonaute 2 (Ago2).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Ago 2, also known as eukaryotic translation initiation factor 2C, 2 (EIF2C 2), MGC3183 and Q10.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.
      Gene Symbol
      • EIF2C2
      • AGO2
      • Q10
      • PPD
      • MGC3183
      • Argonaute-2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UKV8 # Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi) (By similarity).
      SIZE: 851 amino acids; 96799 Da
      SUBUNIT: Interacts with DICER1 through its Piwi domain.
      SUBCELLULAR LOCATION: Cytoplasm, P-body. Note=Cytoplasmic bodies.
      DOMAIN:SwissProt: Q9UKV8 Piwi domain is thought to provide RNA cleavage activity with a mechanism similar to RNase H (By similarity).
      SIMILARITY: Belongs to the argonaute family. & Contains 1 PAZ domain. & Contains 1 Piwi domain.
      Molecular Weight100 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot in RIPA and cytosolic lysates from HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ago 2 (rabbit polyclonal IgG) 2991975
      Anti-Ago 2 (rabbit polyclonal IgG) Q2922231
      Anti-Ago 2 (rabbit polyclonal IgG) - 2026215 2026215
      Anti-Ago 2 (rabbit polyclonal IgG) - 2149493 2149493
      Anti-Ago 2 (rabbit polyclonal IgG) - 2325114 2325114
      Anti-Ago 2 - 2446911 2446911
      Anti-Ago 2 - 26966 26966
      Anti-Ago 2 - 32488 32488
      Anti-Ago 2 - DAM1713231 DAM1713231
      Anti-Ago 2 - DAM1764454 DAM1764454

      References

      Reference overviewApplicationPub Med ID
      Human immunodeficiency virus Tat associates with a specific set of cellular RNAs.
      Bouwman, RD; Palser, A; Parry, CM; Coulter, E; Rasaiyaah, J; Kellam, P; Jenner, RG
      Retrovirology  11  53  2014

      Show Abstract
      24990269 24990269
      Identification of distinct miRNA target regulation between breast cancer molecular subtypes using AGO2-PAR-CLIP and patient datasets.
      Farazi, TA; Ten Hoeve, JJ; Brown, M; Mihailovic, A; Horlings, HM; van de Vijver, MJ; Tuschl, T; Wessels, LF
      Genome biology  15  R9  2014

      Show Abstract
      Western Blotting24398324 24398324
      High-resolution profiling and analysis of viral and host small RNAs during human cytomegalovirus infection.
      Stark, TJ; Arnold, JD; Spector, DH; Yeo, GW
      Journal of virology  86  226-35  2012

      Show Abstract
      22013051 22013051
      Two-step cleavage of hairpin RNA with 5' overhangs by human DICER.
      Ando, Y; Maida, Y; Morinaga, A; Burroughs, AM; Kimura, R; Chiba, J; Suzuki, H; Masutomi, K; Hayashizaki, Y
      BMC molecular biology  12  6  2011

      Show Abstract Full Text Article
      21306637 21306637
      Limiting Ago protein restricts RNAi and microRNA biogenesis during early development in Xenopus laevis.
      Lund, E; Sheets, MD; Imboden, SB; Dahlberg, JE
      Genes & development  25  1121-31  2011

      Show Abstract
      21576259 21576259
      A role for human Dicer in pre-RISC loading of siRNAs.
      Sakurai, K; Amarzguioui, M; Kim, DH; Alluin, J; Heale, B; Song, MS; Gatignol, A; Behlke, MA; Rossi, JJ
      Nucleic acids research  39  1510-25  2011

      Show Abstract
      20972213 20972213
      A comprehensive survey of 3' animal miRNA modification events and a possible role for 3' adenylation in modulating miRNA targeting effectiveness.
      Burroughs, AM; Ando, Y; de Hoon, MJ; Tomaru, Y; Nishibu, T; Ukekawa, R; Funakoshi, T; Kurokawa, T; Suzuki, H; Hayashizaki, Y; Daub, CO
      Genome research  20  1398-410  2010

      Show Abstract
      20719920 20719920
      Transcriptional regulation by small RNAs at sequences downstream from 3' gene termini.
      Yue, X; Schwartz, JC; Chu, Y; Younger, ST; Gagnon, KT; Elbashir, S; Janowski, BA; Corey, DR
      Nature chemical biology  6  621-9  2010

      Show Abstract
      20581822 20581822
      The expression of Argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts.
      Donker, RB; Mouillet, JF; Nelson, DM; Sadovsky, Y
      Molecular human reproduction  13  273-9  2007

      Show Abstract
      17327266 17327266
      Involvement of AGO1 and AGO2 in mammalian transcriptional silencing
      Janowski, Bethany A, et al
      Nat Struct Mol Biol, 13:787-92 (2006)  2006

      16936728 16936728

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies