04-642 | Anti-Ago2 Antibody, clone 9E8.2

04-642
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ChIP, ChIP-seqMAscitesMonoclonal Antibody
      Description
      Catalogue Number04-642
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Ago2 Antibody, clone 9E8.2
      Alternate Names
      • Argonaute-2
      • Eukaryotic translation initiation factor 2C
      • AGO2
      • eIF-2C
      • Slicer protein
      • MGC3183
      • Piwi domain protein
      • PPD
      Background InformationAgo2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).
      References
      Product Information
      FormatAscites
      PresentationAscites with 0.05% sodium azide
      Applications
      ApplicationAnti-Ago2 Antibody, clone 9E8.2 is a Mouse Monoclonal Antibody for detection of Ago2 also known as Argonaute-2, Eukaryotic translation initiation factor 2C & has been validated in WB and has been demonstrated to perform in ChIP and ChIP-seq.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      Application NotesChIP and ChIP-seq: A representative lot of this antibody was used to perform ChIP and ChIP-seq ( Woolnough, JL, Atwood, BL, and Giles KE (2015), MCB Vol. 35 No. 13, p. 2278-2294).
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence YSGAGPALAPPAPPPPIQG
      Epitopenear the N-terminus of Ago2
      Clone9E8.2
      HostMouse
      Specificityhuman Ago2
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.
      Gene Symbol
      • Ago-2
      • EIF2C2
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P45973 # Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Can interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
      SIZE: 191 amino acids; 22225 Da
      SUBUNIT: Interacts with SUV420H1 and SUV420H2 (By similarity). Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with MIS12 and C20orf127.
      SUBCELLULAR LOCATION: Nucleus. Centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
      PTM: Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper- phosphorylated during mitosis.
      SIMILARITY: SwissProt: P45973 ## Contains 2 chromo domains.
      Molecular Weight~100 kda
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ago2, clone 9E8.2 - 2136995 2136995
      Anti-Ago2, clone 9E8.2 - 2344738 2344738
      Anti-Ago2, clone 9E8.2 - 2464567 2464567
      Anti-Ago2, clone 9E8.2 - 1970728 1970728
      Anti-Ago2, clone 9E8.2 - 2033666 2033666
      Anti-Ago2, clone 9E8.2 - 2110563 2110563
      Anti-Ago2, clone 9E8.2 - 2246833 2246833
      Anti-Ago2, clone 9E8.2 - DAM1606785 DAM1606785
      Anti-Ago2, clone 9E8.2 - NG1526827 NG1526827
      Anti-Ago2, clone 9E8.2 - NG1660987 NG1660987

      References

      Reference overviewPub Med ID
      PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins.
      Spitzer, J; Hafner, M; Landthaler, M; Ascano, M; Farazi, T; Wardle, G; Nusbaum, J; Khorshid, M; Burger, L; Zavolan, M; Tuschl, T
      Methods in enzymology  539  113-61  2014

      Show Abstract
      24581442 24581442
      Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells.
      Manavalan, TT; Teng, Y; Appana, SN; Datta, S; Kalbfleisch, TS; Li, Y; Klinge, CM
      Cancer letters  313  26-43  2011

      Show Abstract
      21955614 21955614

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

      Data Sheet

      Title
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies