05-591 | Anti-Akt/PKB Antibody, PH Domain, clone SKB1

05-591
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, ICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-591
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Akt/PKB Antibody, PH Domain, clone SKB1
      Background InformationAkt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: Non-Stim A431 Cell Lysate (12-301)
      PresentationProtein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.02 M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide and 30% glycerol.
      Applications
      ApplicationDetect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1 validated for use in FC, IC, IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST-fusion protein corresponding to residues 1-149 of human Akt1
      CloneSKB1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Akt1/PKBα.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • MGC99656
      • RAC-ALPHA
      • RAC-PK-alpha
      • C-AKT
      • PKB
      • AKT
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from A431 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Akt/PKB, PH Domain, clone SKB1 - 2123701 2123701
      Anti-Akt/PKB, PH Domain, -2557589 2557589
      Anti-Akt/PKB, PH Domain, -2603798 2603798
      Anti-Akt/PKB, PH Domain, -2626023 2626023
      Anti-Akt/PKB, PH Domain, -2654216 2654216
      Anti-Akt/PKB, PH Domain, -2697425 2697425
      Anti-Akt/PKB, PH Domain, -2759752 2759752
      Anti-Akt/PKB, PH Domain, -2798557 2798557
      Anti-Akt/PKB, PH Domain, clone SKB1 (mouse ascites) - DAM1475050 DAM1475050
      Anti-Akt/PKB, PH Domain, clone SKB1 - 1988743 1988743

      References

      Reference overviewApplicationPub Med ID
      Cardiomyocyte-specific BMAL1 plays critical roles in metabolism, signaling, and maintenance of contractile function of the heart.
      Young, ME; Brewer, RA; Peliciari-Garcia, RA; Collins, HE; He, L; Birky, TL; Peden, BW; Thompson, EG; Ammons, BJ; Bray, MS; Chatham, JC; Wende, AR; Yang, Q; Chow, CW; Martino, TA; Gamble, KL
      Journal of biological rhythms  29  257-76  2014

      Show Abstract
      Western Blotting25238855 25238855
      Interrogating signaling nodes involved in cellular transformations using kinase activity probes.
      Stains, CI; Tedford, NC; Walkup, TC; Luković, E; Goguen, BN; Griffith, LG; Lauffenburger, DA; Imperiali, B
      Chemistry & biology  19  210-7  2012

      Show Abstract
      22365604 22365604
      Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer.
      Jones, LW; Antonelli, J; Masko, EM; Broadwater, G; Lascola, CD; Fels, D; Dewhirst, MW; Dyck, JR; Nagendran, J; Flores, CT; Betof, AS; Nelson, ER; Pollak, M; Dash, RC; Young, ME; Freedland, SJ
      Journal of applied physiology (Bethesda, Md. : 1985)  113  263-72  2012

      Show Abstract
      Western Blotting22604887 22604887
      Resveratrol helps recovery from fatty liver and protects against hepatocellular carcinoma induced by hepatitis B virus X protein in a mouse model.
      Lin, HC; Chen, YF; Hsu, WH; Yang, CW; Kao, CH; Tsai, TF
      Cancer prevention research (Philadelphia, Pa.)  5  952-62  2012

      Show Abstract
      22659145 22659145
      Delayed treatment with a novel neurotrophic compound reduces behavioral deficits in rabbit ischemic stroke.
      Lapchak, PA; Schubert, DR; Maher, PA
      Journal of neurochemistry  116  122-31  2011

      Show Abstract Full Text Article
      21054387 21054387
      Thermogenic activation induces FGF21 expression and release in brown adipose tissue.
      Hondares E, Iglesias R, Giralt A, Gonzalez FJ, Giralt M, Mampel T, Villarroya F
      J Biol Chem  2011

      Show Abstract
      21317437 21317437
      Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo.
      Pulinilkunnil, T; He, H; Kong, D; Asakura, K; Peroni, OD; Lee, A; Kahn, BB
      The Journal of biological chemistry  286  8798-809  2011

      Show Abstract
      21209093 21209093
      Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle.
      Treebak, JT; Taylor, EB; Witczak, CA; An, D; Toyoda, T; Koh, HJ; Xie, J; Feener, EP; Wojtaszewski, JF; Hirshman, MF; Goodyear, LJ
      American journal of physiology. Cell physiology  298  C377-85  2010

      Show Abstract Full Text Article
      Western Blotting19923418 19923418
      DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival.
      Peterson, Timothy R, et al.
      Cell, 137: 873-86 (2009)  2009

      Show Abstract
      19446321 19446321
      Therapeutically targeting ErbB3: a key node in ligand-induced activation of the ErbB receptor-PI3K axis.
      Birgit Schoeberl,Emily A Pace,Jonathan B Fitzgerald,Brian D Harms,Lihui Xu,Lin Nie,Bryan Linggi,Ashish Kalra,Violette Paragas,Raghida Bukhalid,Viara Grantcharova,Neeraj Kohli,Kip A West,Magdalena Leszczyniecka,Michael J Feldhaus,Arthur J Kudla,Ulrik B Nielsen
      Science signaling  2  2009

      Show Abstract
      19567914 19567914

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      Alternative Format

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      16-293 Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 488 Show Pricing & Availability

      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies