05-591-AF647 | Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 647

05-591-AF647
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RICCMAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue Number05-591-AF647
      DescriptionAnti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 647
      Alternate Names
      • RAC-alpha serine/threonine-protein kinase
      • PKB
      • PKB alpha
      • Protein kinase B
      • Protein kinase B alpha
      • Proto-oncogene c-Akt
      • RAC-PK-alpha
      Background InformationRAC-alpha serine/threonine-protein kinase (EC 2.7.11.1; UniProt P31749; also known as PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha) is encoded by the AKT1 (also known as PKB, RAC) gene (Gene ID 207) in human. AKTs (protein kinase Bs) are serine/threonine kinases that play an important role in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. There exit three types of AKTs (α, β, γ or AKT 1, 2, 3) sharing a high degree of homology, but differing slightly in the localization of their regulatory phosphorylation sites. AKT α is the predominant isoform in most tissues, whereas the highest expression of AKT β is observed in the insulin-responsive tissues, and AKT γ is abundant in brain tissue. Each AKT type is composed of three functionally distinct regions, including an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified mouse monoclonal IgG antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Applications
      ApplicationThis Anti-Akt Antibody, PH Domain, clone SKB1, Alexa Fluor® 647 is validated for use in Immunocytochemistry for the detection of PKB.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Akt/PKB in A431 cells.
      The unconjugated antibody (Cat. No. 05-591) is shown to be suitable also for flow cytometry, immunocytochemistry, immunoprecipitation, and Western blotting applications.
      Biological Information
      ImmunogenGST-fusion protein corresponding to residues 1-149 of human Akt1.
      EpitopeAmino acids 1-149
      CloneSKB1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone SKB1 recognizes Akt1 and Akt2, but not Akt3, GST fusion proteins at 100 ng by immunoblotting analysis using the unconjugated antibody (Cat. No. 05-591).
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteMouse, Human, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AKT1
      • PKB
      • RAC
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~60 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Akt/PKB in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies