06-885 | Anti-Akt1/PKBα Antibody, PH domain,

06-885
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-885
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Akt1/PKBα Antibody, PH domain,
      References
      Product Information
      FormatAffinity Purified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 30% glycerol
      Applications
      ApplicationAnti-Akt1 Antibody, PH domain, is an antibody against Akt1/PKBα for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide (FHVETPEEREEWT-C) corresponding to amino acids 88-100 of human Akt1/PKBα Pleckstrin Homology Domain
      HostRabbit
      SpecificityAkt1/PKBα
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • RAC-ALPHA
      • PKB
      • MGC99656
      • RAC-PK-alpha
      • C-AKT
      • AKT
      Purification MethodImmunoaffinity Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight60kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Akt1/PKBa, PH Domain - DAM1493350 DAM1493350
      Anti-Akt1/PKBa, PH Domain - JBC1870065 JBC1870065
      Anti-Akt1/PKBalpha;, PH domain, polyclonal - 17538 17538
      Anti-Akt1/PKBalpha;, PH domain, polyclonal - 25332 25332
      Anti-Akt1/PKBalpha;, PH domain, polyclonal - 27708 27708
      Anti-Akt1/PKBalpha;, PH domain, polyclonal - 32482 32482

      References

      Reference overviewPub Med ID
      Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily.
      Jones, P F, et al.
      Proc. Natl. Acad. Sci. U.S.A., 88: 4171-5 (1991)  1991

      Show Abstract
      1851997 1851997

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies