04-771 | Anti-Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal

04-771
100 µl  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IPRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-771
      Replaces04-406
      DescriptionAnti-Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal
      Alternate Names
      • AKT2 kinase
      • Murine thymoma viral (v-akt) homolog-2
      • PKB beta
      • Protein kinase Akt-2
      • Protein kinase B, beta
      • rac protein kinase beta
      • v-akt murine thymoma viral oncogene homolog 2
      Background InformationAkt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3 (which is inactivated by this phosphorylation), and the FoxO family of transcription factors (PRAS40, AS160).
      References
      Product Information
      FormatPurified
      Control
      • L6 cell lysate
      PresentationCultured supernantant containing 0.05% sodium azide.
      Applications
      ApplicationAnti-Akt2/PKBβ Antibody, clone AW114 detects level of Akt2/PKBβ & has been published & validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (CRYDSLGSLELDQRTH) corresponding to amino acids 455-469 of human Akt2/PKBβ, with an N-terminal cysteine added for conjugation purposes.
      Epitopea.a. 455-469
      CloneAW114
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Akt2/PKBβ, Mr ~60 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman and rat. Predicted to cross-react with mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
      Gene Symbol
      • EC 2.7.11.1
      • PKBB
      • PKBBETA
      • PRKBB
      • RAC-BETA
      • RAC-PK-beta
      UniProt Number
      UniProt SummaryFUNCTION: General protein kinase capable of phosphorylating several known proteins.
      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
      ENZYME REGULATION:Two specific sites, one in the kinase domain (Thr-309) and the other in the C-terminal regulatory region (Ser-474), need to be phosphorylated for its full activation.
      SUBUNIT STRUCTURE: Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B.
      TISSUE SPECIFICITY:In all human cell types so far analyzed.
      INVOLVEMENT IN DISEASE: Alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
      Molecular Weight~60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: A 1:500 dilution of this antibody detected Akt2/PKBβ in HepG2 lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µl
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

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      05-771 Anti-Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal Show Pricing & Availability
      07-372 Anti-Akt2/PKBβ Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies