05-831-I | Anti-Amyloid Beta (ABeta) x-42 Antibody, clone 12F4

05-831-I
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHC, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-831-I
      Replaces05-831
      DescriptionAnti-Amyloid Beta (ABeta) x-42 Antibody, clone 12F4
      Alternate Names
      • Amyloid beta A4 protein
      • ABPP
      • APPI
      • APP
      • Alzheimer disease amyloid protein
      • Cerebral vascular amyloid peptide
      • CVAP
      • PreA4
      • Protease nexin-II
      • PN-II
      Background InformationOne of the most important and initial steps which causes loss of memory and cognition in Alzheimer’s Disease (AD) involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 amino acid peptides (Amyloid Beta) 1-40, 1-42 and 1-43). Polymerization of beta-amyloid and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.
      References
      Product Information
      FormatPurified
      Control
      • Human Alzheimer's brain tissue.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Amyloid Beta (ABeta) x-42, clone 12F4 is an antibody targeting the Amyloid Beta (ABeta) x-42 protein, validated for use in IHC & ELISA.
      Key Applications
      • Immunohistochemistry
      • Enzyme Immunoassay (ELISA)
      Application NotesImmunohistohemistry Analysis: A 1:500 dilution from a representative lot detected Amyloid Beta (ABeta) x-42 in human pons/midbrain tissue.

      ELISA Analysis: A representative lot detected Amyloid Beta (ABeta) x-42, but demonstrates a loss of signal against Amyloid Beta (ABeta) x-42.

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Amyloid Beta (ABeta) x-42, but demonstrated a loss of signal against x-40 and not x-43 in WB (Prof. J. Buxbaum, Mt. Sinai School of Medicine).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Amyloid Beta (ABeta) x-42.
      Clone12F4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody detects Amyloid Beta (ABeta) x-42, but does not cross react with Amyloid Beta (ABeta) x-40 or Amyloid Beta (Abeta) x-43.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APP
      • A4
      • AD1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight4 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human Alzheimer's disease brain tissue.

      Immunohistochemistry Analysis: A 1:2,000 dilution of this antibody detected Amyloid Beta (Abeta) x-42 in human Alzheimer's disease brain tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Amyloid Beta (ABeta) x-42, clone 12F4 - Q2129762 Q2129762
      Anti-Amyloid Beta (ABeta) x-42, -2520499 2520499
      Anti-Amyloid Beta (ABeta) x-42, -2547766 2547766
      Anti-Amyloid Beta (ABeta) x-42, -2548731 2548731
      Anti-Amyloid Beta (ABeta) x-42, -2567139 2567139
      Anti-Amyloid Beta (ABeta) x-42, -2583433 2583433
      Anti-Amyloid Beta (ABeta) x-42, -2616196 2616196
      Anti-Amyloid Beta (ABeta) x-42, -2651999 2651999
      Anti-Amyloid Beta (ABeta) x-42, -2768480 2768480

      Newsletters / Publications

      Title
      Research Focus - Volume 2, 2013